Sample
serum, plasma tissues homogenates, cell culture supernatants, other biological fluids
Detection Method
Sandwich ELISA
Intended Use
The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PLGF in human serum, plasma tissue homogenates, cell culture supernates and other biological fluids.
Contents of Kit
1. Pre-Coated, ready to use 96-well strip plate: 1
2. Plate sealer for 96 wells: 4
3. Standard (freezedried): 2
4. Standard Diluent: 1 x 20 mL
5. Detection Reagent A: 1 x 120 μL
6. Assay Diluent A (2X concentrate): 1 x 6 mL
7. Detection Reagent B: 1 x 120 μL
8. Assay Diluent B (2X concentrate): 1 x 6 mL
9. TMB Substrate: 1 x 9 mL
10. Stop Solution: 1 x 6 mL
11. Wash Buffer (30X concentrate): 1 x 20 mL
Storage
All the reagents should be kept according to the labels on vials. For more detailed information, please download the following document on our website.
Citations
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Kodaman, PH; Behrman, HR; et al. Endocrine-regulated and protein kinase C-dependent generation of superoxide by rat preovulatory follicles. ENDOCRINOLOGY 142:687-693(2001).
Mondal, M; Meyer, HHD; et al. Highly sensitive second antibody format enzymeimmunoassay for determination of 13,14-dihydro-15-keto-PGF(2 alpha) in blood plasma of mithun (Bos frontalis). PROSTAGLANDINS & OTHER LIPID MEDIATORS 80:100-109(2006).