Human Micro-Albumin ELISA Kit

Sample

Urine

Species Reactivity

Human

Detection Method

cELISA

Intended Use

This Kit is a test system for the quantitative measurement human Albumin in urine.

Contents of Kit

1. 1 One divisible microplate consisting of 12 modules of 8 wells each. Ready to use.
2. 1×1.5 mL Calibrator A 0.15 μg/mL, containing serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use.
3. 1×1.5 mL Calibrator B 1.5 μg/mL, containing albumin in a serum/buffer matrix (PBS, detergent, NaN₃ 0.09%), yellow. Ready to use.
4. 1×1.5 mL Calibrator C 6 μg/mL, containing albumin in a serum/buffer matrix (PBS, detergent, NaN₃ 0.09%), yellow. Ready to use.
5. 1×1.5 mL Calibrator D 25 μg/mL, containing albumin in a serum/buffer matrix (PBS, detergent, NaN₃ 0.09%), yellow. Ready to use.
6. 1×1.5 mL Calibrator E 100 μg/mL, containing albumin in a serum/buffer matrix (PBS, detergent, NaN₃ 0.09%), yellow. Ready to use.
7. 1×1.5 mL Calibrator F 400 μg/mL, containing albumin in a serum/buffer matrix (PBS, detergent, NaN₃ 0.09%), yellow. Ready to use.
8. 1×1.5 mL Control positive, containing albumin in a serum/buffer matrix (PBS, detergent, NaN₃ 0.09%), yellow. Ready to use. The concentration is specified on the certificate of analysis.
9. 1×1.5 mL Control negative, containing albumin in a serum/buffer matrix (PBS, detergent, NaN₃ 0.09%), yellow. Ready to use. The concentration is specified on the certificate of analysis.
10. 20 mL Sample Buffer PA, containing PBS, detergent, preservative sodium azide 0.09%, yellow. Ready to use.
11. 15 mL Enzyme Conjugate; containing anti-human albumin antibodies, HRP labelled; PBS, BSA, detergent, preservative Proclin 0.05%, light red. Ready to use.
12. 15 mL TMB Substrate; containing 3,3', 5,5'-Tetramethylbenzidin, colorless. Ready to use.
13. 15 mL Stop Solution; contains acid. Ready to use.
14. 20 mL Wash Buffer, containing Tris, detergent, preservative sodium azide 0.09%; 50× conc.
15. 1 Instruction for Use
16. 1 Certificate of Analysis

Storage

1. Collect morning urine.
2. Samples may be refrigerated at 2°C - 8°C for up to five days or stored at -20°C up to six months.
3. Avoid repetitive freezing and thawing of urine samples.
4. Store test kit at 2°C - 8°C in the dark.
5. Do not expose reagents to heat, sun, or strong light during storage and usage.
6. Store microplate sealed and desiccated in the clip bag provided.
7. Shelf life of the unopened test kit is 18 months from day of production. Unopened reagents are stable until expiration of the kit. See labels for individual batch.
8. Diluted Wash Buffer and Sample Buffer are stable for at least 30 days when stored at 2°C - 8°C. We recommend consumption on the same day.

Detection Range

1.5 - 400 μg/mL

Detection Limit

0.5 μg/mL

General Description

Proteins passing the glomerular basal membrane of the kidney undergo differentiated filtering. The permeability is inversely proportional to the molecular weight (albumin about 0.6 %, myoglobulin about 75%). Nevertheless, only minimal quantities of protein are detectable in urine, because big quantities of protein are reabsorbed by the tubuli. Elevated glomerular protein permeability and high tubular plasma protein elimination can be differentiated by measuring the molecular weight distribution of the eliminated proteins.
The pattern of eliminated proteins in urine give information about :
- elevated protein elimination
- differentiation of proteinuria
- prediagnosis of a kidney defect
- glomerular or tubular proteinuria
Diagnostically relevant proteins are:
- IgG (150 kD)
- Albumin (66 kD)
- Alpha-1-Microglobulin (33 kD)
- Retinol binding protein (21 kD)
- Beta-2-Microglobulin (12 kD)
- Immunoglobulin light chains (Bence-Jones protein) ( 22 kD)
Albumin has a relative molecular mass of 66 kDa. It is contained in urine at very low concentrations. In case of a very active glomerular filtering process the albumin secretion can arise without an underlying kidney disease. This situation is called "microalbuminuria". The detection of these small secretion quantities (30 to 150 μg/min or mL) requires very sensitive test systems. immunological techniques. Physical stress can also induce elevated albumin secretion, without the occurrence of a kidney disease.
In diabetes, albumin secretion is a very important parameter for the evaluation of the kidney function. Urine values higher than 25 μg/mL indicate a detrimental kidney function of insulin dependent (type I) and non-insulin-dependent (type II) diabetic patients. The determination of albumin is therefore an important diagnostic tool in diabetic nephropathies.