Human MHC Class I Polypeptide-related Sequence B, MICB ELISA Kit

Species Reactivity

Human

Detection Method

Sandwich ELISA

Intended Use

The human MICB ELISA kit is for the quantitative determination of human MICB.
This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on five 96-well plates.

Contents of Kit

1. Bring all reagents to room temperature before use.
2. Capture Antibody: 0.5 mg/mL of mouse anti-MICB monoclonal antibody.
3. Detection Antibody: Each vial contains 70 & mu;g biotinylated rabbit anti-MICB polyclonal antibody. Reconstitute with 1 mL detection antibody dilution buffer.
4. Standard: Each vial contains 50ng of recombinant MICB.
5. Streptavidin-HRP: 50 μL of streptavidin conjugated to horse-radish-peroxidase. 1:2,000 Dilution in detection antibody dilution buffer before use.

Storage

Keep streptavidin-HRP at 4°C and protect it from prolonged exposure to light. Aliquot all other reagents and store at -20°C to -70°C in a manual defrost freezer.

Sensitivity

The minimum detectable dose of human MICB was determined to be approximately 62.5 pg/mL. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.