Human minichromosome maintenance complex component 5 Cell-Based ELISA Kit

Species Reactivity

Human

Intended Use

The MCM5 (Human) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor MCM5 protein expression profile in cells. The kit can be used for measuring the relative amounts of MCM5 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on MCM5.

Storage

Upon receipt, the kit should be stored at 4°C. The un-opened kit will be stable for up to 6 months from the date of shipment if stored at 4°C. Diluted Anti-MCM5 Antibody and diluted Anti-GAPDH Antibody can each be stored at 4°C for up to two weeks. HRP-Conjugated Anti-Rabbit IgG and HRP-Conjugate Anti-Mouse IgG will be stable at 4°C for up to six months. The 1% SDS Solution should be stored at room temperature or warmed up to room temperature if stored at 4°C.

General Description

DNA replication licensing factor MCM5 is a protein that in humans is encoded by the MCM5 gene. The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.