Human Interleukin 21, IL21 ELISA Kit

Name: Human Interleukin 21, IL21 ELISA Kit
SKU: DEIA161
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

serum, plasma, other biological fluids

Species Reactivity

Human

Detection Method

Sandwich ELISA

Intended Use

This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay(ELISA). It is developed for quantitative measurement of Human IL21 in serum, plasma and other biological fluids.

Contents of Kit

1. Aluminium pouches with a Microwell Plate coated with antibody to human IL-21 (8 x 12)
2. 2 vials human IL-21 Standard lyophilized, 1000 pg/mL upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human IL-21 antibody
4. 2 vials Streptavidin-HRP solution
5. 1 bottle Standard/sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20X (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10.1 vial Stop Solution

Storage

Store at 2-8°C. For more detailed information, please download the following document on our website.

Detection Range

31.25-2000 pg/mL

Citations

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Interleukin-21 plays a critical role in the pathogenesis and severity of type I autoimmune hepatitis

Kazumichi Abe, Atsushi Takahashi, Hiromichi Imaizumi, Manabu Hayashi, Ken Okai, Yukiko Kanno, Hiroshi Watanabe & Hiromasa Ohira

SpringerPlus2016 JunePubMed ID: 27386263Read Article

Applications: ELISA
Reactive species: Human
"Abstract: Background and aims: Recently, the number of follicular helper T (Tfh) cells expressing interleukin (IL)-21 was found to increase in peripheral blood of human and murine models of autoimmune hepatitis (AIH). IL-21, the most recently discovered member of the type-I cytokine family, exerts various effects on the immune system, including B cell activation, plasma cell differentiation, and immunoglobulin production. We aimed to assess the relationship of serum IL-21 levels in patients with type I AIH with clinical and laboratory parameters and histology."
Article snippet: ELISA kits were used to determine the levels of serum IL-21 (Creative Diagnostics, NY, USA), IL-18 (*), CCL20, CXCL9 (*), CCR6 (*), and CXCR3 (*), according to the manufacturer’s instructions.

Patients with AIH have higher serum IL-21 levels than those with other liver diseases and healthy controls. p values were calculated with the Mann–Whitney test. (Kazumichi Abe, <i>et al</i>, 2016)

Figure 1. Patients with AIH have higher serum IL-21 levels than those with other liver diseases and healthy controls. p values were calculated with the Mann–Whitney test.

Increased ratio of ICOS(+) /PD-1(+) follicular helper T cells positively correlates with the development of human idiopathic membranous nephropathy

Xu Shi, Zhihui Qu, Li Zhang, Nan Zhang, Yong Liu, Man Li, Jinpeng Qiu, Yanfang Jiang

Clin Exp Pharmacol Physiol2016 AprPubMed ID: 26845249Read Article

Applications: ELISA
Reactive species: Human
"Abstract: To identify the frequencies of different subsets of peripheral blood follicular helper T (Tfh) cells in human idiopathic membranous nephropathy (IMN), 39 patients with new onset IMN and 18 age- and gender-matched healthy controls (HC) were enrolled for this study. The frequency of Tfh cells in venous blood were measured by flow cytometry, while concentration of serum IL-21 was detected by enzyme-linked immunosorbent assay. Correlation between the clinical features of IMN and Tfh cells was assessed by Spearman's rank correlation test. Overall, the frequencies of total, ICOS(+) , and PD-1(+) Tfh cells were increased in IMN patients, while the ratio of ICOS(+) /PD-1(+) Tfh cells positively correlated with IMN progression. However, the elevated serum IL-21 level in three subgroups of IMN patients, stratified based on 24-h urine protein levels, was not statistically significant compared to HC. Nonetheless, intracellular IL-21 in Tfh cells was generally increased in all IMN patients, and closely correlated with IMN development. Finally, the frequency of IL-21(+) Tfh cells and the ratio of ICOS(+) /PD-1(+) Tfh cells were positively correlated with the estimated 24-h urine protein of IMN patients. The data indicated that Tfh cells contribute to the pathogenicity of IMN. The ratio of ICOS(+) /PD-1(+) Tfh cells and the frequency of IL-21(+) Tfh cells may be indicators for evaluating the IMN development."
Article snippet: The concentrations of serum IL-21 in individual subjects were determined by enzyme-linked immunosorbent assay (ELISA) using human IL-21 ELISA kit, according to the manufacturers’ instruction (Creative Diagnostics, New York, NY, USA).

Serum IL21 concentration. (Xu Shi, <i>et al</i>, 2016)

Figure 1. The concentration of serum IL-21 detected by enzymelinked immunosorbent assay (ELISA)

Documents

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References

Interleukin 21 and Its Receptor Play a Role in Proliferation, Migration and Invasion of Breast Cancer Cells

CANCER GENOMICS & PROTEOMICS

Authors: Wang, Li-Na; Cui, Yu-Xin; Ruge, Fiona; Jiang, Wen G.

Abstract

Interleukin 21 (IL21) is a cytokine produced predominantly by cluster of differentiation 4 (CD4+) T-cells and natural killer T-cells. There exists evidence that IL21 is implicated in various immunological processes through its specific receptor (IL21R). However, the participation of IL21 in the pathogenesis of solid tumors is not fully conclusive. In the present study, we demonstrated that there was differential expression of IL21R in breast cancer cells using reverse transcription-polymerase chain reaction (RT-PCR), western blotting and sequence analysis. The expression of IL21R was stronger in MDA-231 cells, weaker in MCF7 but negative in ZR-75.1 cells. The invasion and migratory capacity of IL21R+ MDA-231 cells was enhanced by IL21 in a dose-dependent manner. After IL21R was knocked-down by siRNA gene silencing, the response of MDA-231 to treatment with IL21 was attenuated. We found that siRNA silencing of IL21R also spontaneously suppressed cell proliferation. However, IL21 had no additional effect on the proliferation of MDA-231 cells. We also found that IL21R was involved in signaling pathways of matrix metalloproteinases (MMPs), that are crucial for spreading and migration of metastatic MDA231 cells. In conclusion, we unveiled the roles of IL21R in breast cancer cells, which enhances our knowledge on immunological regulation of cancer cells through the axis of IL21 and its receptor.

The Role of IL-17 Promotes Spinal Cord Neuroinflammation via Activation of the Transcription Factor STAT3 after Spinal Cord Injury in the Rat

MEDIATORS OF INFLAMMATION

Authors: Zong, Shaohui; Zeng, Gaofeng; Fang, Ye; Peng, Jinzhen; Tao, Yong; Li, Keke; Zhao, Jingmin

Abstract

Study Design. In this study, we investigated the role of IL-17 via activation of STAT3 in the pathophysiology of SCI. Objective. The purpose of the experiments is to study the expression of IL-17 and related cytokines via STAT3 signaling pathways, which is caused by the acute inflammatory response following SCI in different periods via establishing an acute SCI model in rat. Methods. Basso, Beattie, and Bresnahan hind limb locomotor rating scale was used to assess the rat hind limb motor function. Immunohistochemistry was used to determine the expression levels of IL-17 and p-STAT3 in spinal cord tissues. Western blotting analysis was used to determine the protein expression of p-STAT3 in spinal cord tissue. RT-PCR was used to analyze the mRNA expression of IL-17 and IL-23p19 in the spleen tissue. ELISA was used to determine the peripheral blood serum levels of IL-6, IL21, and IL-23. Results. Compared to the sham-operated group, the expression levels of IL-17, p-STAT3, IL-6, IL-21, and IL-23 were significantly increased and peaked at 24 h after SCI. The increased levels of cytokines were correlated with the SCI disease stages. Conclusion. IL-17 may play an important role in promoting spinal cord neuroinflammation after SCI via activation of STAT3.