Human Interleukin 10 Receptor, Beta, IL10RB ELISA Kit

Species Reactivity

Human

Detection Method

Sandwich ELISA

Intended Use

The human IL10RB ELISA kit is for the quantitative determination of human IL10RB.
This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on five 96-well plates.

Contents of Kit

1. Bring all reagents to room temperature before use.
2. Capture Antibody: 0.5 mg/mL of mouse anti-IL10RB monoclonal antibody.
3. Detection Antibody: 0.7 mg/mL of rabbit anti-IL10RB polyclonal antibody conjugated to horseradish-peroxidase(HRP). Dilute to a working concentration of 1.0 μg/mL in detection antibody dilution buffer before use.
4. Standard: Each vial contains 110 ng of recombinant IL10RB. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C to -70°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5,000 pg/mL is recommended.

Storage

Detection Antibody should be protected from prolonged exposure to light. Aliquot all other reagents and store at -20°C to -70°C in a manual defrost freezer.

Sensitivity

The minimum detectable dose of human IL10RB was determined to be approximately 78.125 pg/mL. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.