Human Interleukin 10 Receptor, Beta, IL10RB ELISA Kit

Sample

Plasma, tissue homogenates and other biological fluids.

Species Reactivity

Human

Detection Method

sELISA

Intended Use

The human IL10RB / CRFB4 ELISA Pair Set is for the quantitative determination of human IL10RB / CRFB4.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.

Contents of Kit

Capture Antibody: 0.5 mg/mL of mouse anti-IL10RB monoclonal antibody. Dilute to a working concentration of 2.0 μg/mL in CBS before coating.
Detection Antibody: 0.7 mg/mL rabbit anti-IL10RB polyclonal antibody conjugated to horseradish-peroxidase ( HRP ). Dilute to a working concentration of 0.5 μg/mL in detection antibody diluteion buffer before use
Standard: Each vial contains 110 ng of recombinant IL10RB. Reconstitute standard powder with 1mL detection antibody dilution buffer. After reconstitution, store at -20°C to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5000 pg/mL is recommended.

Storage

Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4°C and protect it from prolonged exposure to light for up to 6 months from date of receipt. Do not freeze!
Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted standard at -80°C for up to 1 month. Avoid repeated freezethaw cycles.

Sensitivity

The minimum detectable dose of human IL10RB / CRFB4 was determined to be approximately 78.125 pg/ml.
This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

General Description

The functional IL-10 receptor consists of two polypeptide chains, a ligand binding chain IL-10 Rα (IL-10 R1) and a signal transduction molecule known as IL-10 Rβ (IL-10 R2). Both chains of the IL-10 Rare expressed on many hematopoietic cells, including lymphocytes, monocytes and neutrophils, with the IL-10 Rβ chain expressed at higher levels than the IL-10 Rα chain in most cases. Interleukin 10 receptor beta (IL-10 Rβ), also known as CD210b and CRF2-4, is an 80-85 kDa member of the type II cytokine receptor family of proteins and contains two fibronectin type-III domains. The widely expressed IL-10 Rβ serves as a signal transducing accessory chain not only for IL-10, but also for other interleukins including IL-22, -28A, -28B and -29 (plus IL-26 in human) when complexed to the ligand-binding chains. It has been shown that the action of IL-10 delivered through its receptor can affect a variety of immune functions including inhibition of proinflammatory cytokine synthesis and regulation of the growth and function of B cells and antigen presenting cells. The human IL-10 Rβ shares 69% homology to its mouse counterpart in amino acid sequence. Research evidences indicated that defects in IL10RB are associated with susceptibility to hepatitis B virus infection.

Standard Curve