Human Interleukin-8 Xpress Rapid Test

Name: Human Interleukin-8 Xpress Rapid Test
SKU: DTS1002
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Intended Use

It is an immunochromatographic rapid test for the detection of human interleukin-8 in biological fluids, such as urine, serum or plasma. The assay can be potentially used as an aid for diagnosis of primary and secondary infections (e.g., urinary tract infection and neonatal sepsis), as well as certain neoplastic conditions (e.g., bladder cancer), in conjunction with other criteria.

Storage

Store the cards in their original packaging at room temperature. This product is stable for up to 18 months from the certified date. Freezing the strips is not recommended.

Detection Limit

1. The test is for qualitative detection of hIL-8 in human urine, serum, plasma or other biological fluids, but dose not indicate the quantity of hIL-8.
2. The test is for research use only, and should NOT be used for diagnosis.
3. As in case of all rapid tests, a definitive clinical diagnosis should not be based on the result of a single test but should rather be made after all the clinical findings have been evaluated.

Sensitivity

The assay has a sensitivity to detect hIL-8 down to 0.7 ng/mL.

General Description

IL-8 is also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte derived neutrophil chemotactic factor (MDNCF), T lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI), and is a chemotactic factor that attracts neutrophils, basophils, eosinophils and T-cells. Many cell types, includingmonocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce IL-8 in response to a wide variety of proinflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. IL-8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. It is reported to be angiogenic and plays a positive role in tumor metastasis. IL-8 is regarded as one of the earliest biomarkers for infection and inflammation. This assay takes only 5-10 minutes, compared to the conventional ELISA assay that takes several hours.

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Documents

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References

Interplay between Endoplasmic Reticular Stress and Survivin in Colonic Epithelial Cells

CELLS

Authors: Gundamaraju, Rohit; Vemuri, Ravichandra; Chong, Wai Chin; Myers, Stephen; Norouzi, Shaghayegh; Shastri, Madhur D.; Eri, Rajaraman

Abstract

Sustained endoplasmic reticular stress (ERS) is implicated in aggressive metastasis of cancer cells and increased tumor cell proliferation. Cancer cells activate the unfolded protein response (UPR), which aids in cellular survival and adaptation to harsh conditions. Inhibition of apoptosis, in contrast, is a mechanism adopted by cancer cells with the help of the inhibitor of an apoptosis (IAP) class of proteins such as Survivin to evade cell death and gain a proliferative advantage. In this study, we aimed to reveal the interrelation between ERS and Survivin. We initially verified the expression of Survivin in Winnie (a mouse model of chronic ERS) colon tissues by using immunohistochemistry (IHC) and immunofluorescence (IF) in comparison with wild type Blk6 mice. Additionally, we isolated the goblet cells and determined the expression of Survivin by IF and protein validation. Tunicamycin was utilized at a concentration of 10 mu g/mL to induce ERS in the LS174T cell line and the gene expression of the ERS markers was measured. This was followed by determination of inflammatory cytokines. Inhibition of ERS was carried out by 4Phenyl Butyric acid (4PBA) at a concentration of 10 mM to assess whether there was a reciprocation effect. The downstream cell death assays including caspase 3/7, Annexin V, and poly(ADP-ribose) polymerase (PARP) cleavage were evaluated in the presence of ERS and absence of ERS, which was followed by a proliferative assay (EdU click) with and without ERS. Correspondingly, we inhibited Survivin by YM155 at a concentration of 100 nM and observed the succeeding ERS markers and inflammatory markers. We also verified the caspase 3/7 assay. Our results demonstrate that ERS inhibition not only significantly reduced the UPR genes (Grp78, ATF6, PERK and XBP1) along with Survivin but also downregulated the inflammatory markers such as IL8, IL4, and IL6, which suggests a positive correlation between ERS and the inhibition of apoptosis. Furthermore, we provided evidence that ERS inhibition promoted apoptosis in LS174T cells and shortened the proliferation rate. Moreover, Survivin inhibition by YM155 led to a comparable effect as that of ERS inhibition, which includes attenuation of ERS genes and inflammatory markers as well as the promotion of programmed cell death via the caspase 3/7 pathway. Together, our results propose the interrelation between ERS and inhibition of apoptosis assigning a molecular and therapeutic target for cancer treatment.

Surface water extracts impair gene profiles and differentiation in human mesenchymal stem cells

ENVIRONMENT INTERNATIONAL

Authors: Zhang, Zhou; Liu, Wei; Hu, Junxiang; Qu, Yuanyuan; Zhao, Jing; Pan, Yifan; Zhang, Xin; Quan, Xie

Abstract

Low concentrations of pollutants in surface water challenge the assessment of chronic effects on human health. Human bone mesenchymal stem cells (hBMSCs) were employed as a sensitive and relevant in vitro model to evaluate the potential biological effects caused by mixtures of pollutants in surface water. Organic extracts of surface water collected from Hun River inhibited cell viability in a dose-dependent manner. Surface water extracts at noncytotoxic concentrations induced 533 to 1055 differentially expressed genes (DEGs) in hBMSCs after 48 h of exposure. Total of 370 genes were commonly affected by surface water from different sites and accounted for 35-69% of DEGs impaired by individual sample. Pathways related to human diseases, genetic information processing and organismal systems were enriched based on DEGs. Interleukins (IL1B, IL6 and IL8) were affected and involved in most human diseases related pathways. The significantly downregulation of COL1A1 and the variation of rheumatoid arthritis pathway suggested that surface water potentially inhibited osteogenic differentiation of hBMSCs. Clustering analysis and principle component analysis with DEGs distinguish the surface water from tributary and mainstream. The crossing-species comparison of transcriptomic changes identified 923 and 2715 differentially expressed orthologs in hBMSCs and zebrafish, respectively. After the exposure ceased, the followed osteogenic and adipogenic differentiation in hBMSCs for 14 days were inhibited by the treatment of surface water during undifferentiated period, whereas the non-polar fraction exhibited stronger potency in affecting differentiation than the mid to polar fractions. hBMSCs, combining unsupervised transcriptomic technique and specific endpoints test, are promising in screening the health effects of environmental mixtures in surface water.