Human High sensitive Interleukin 15 ELISA Kit

Name: Human High sensitive Interleukin 15 ELISA Kit
SKU: DEIA160
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, plasma, cell culture supernatant and other biological samples

Species Reactivity

Human

Detection Method

sELISA

Intended Use

In vitro quantitative determination of IL-15 concentrations in serum, plasma, cell culture supernatant and other biological samples.

Contents of Kit

The sealed kit can be stored at 2-8 °C. The storage condition for opened kit is specified in the table below:
1. ELISA Microplate(Dismountable), 8×12. Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C.
2. Lyophilized Standard, 2vial. Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C.
3. Biotin-labeled Antibody(Ready to use), 10ml. 2-8°C (Avoid Direct Light)
4. HRP-Streptavidin/HRP-SA(Ready to use), 20ml. 2-8°C (Avoid Direct Light)
5. TMB Substrate, 10ml. 2-8°C (Avoid Direct Light)
6. Sample Dilution Buffer, 20ml. 2-8°C
7. Assay Dilution, 5ml. 2-8°C
8. Biotin System (BS), 120μl. 2-8°C
9. BS Dilution Buffer, 10ml. 2-8°C
10. Stop Solution, 10ml. 2-8°C
11. Wash Buffer(25×), 2vials, 30ml
12. Plate Sealer, 5 pieces
13. Product Description, 1 copy
Note: The liquid reagent bottle contains slightly more reagent than indicated on the label. Please use pipette accurately measure and do proportional dilution.

Storage

2-8°C (for sealed box), please do not freeze! See kit label for expiry date

Performance Characteristics

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Detection Range

0.31-20pg/ml

Sensitivity

0.875pg/ml

General Description

Uniprot ID: P40933
interleukin-15 (IL-15) is a pro-inflammatory protein that plays a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential for the development and survival of NK cells and CD8+ T cells. IL-15 activates the Jak/Stat pathway through heterotrimer receptor signaling. Although IL-15 mRNA is expressed in many cell types, the secreted protein is rarely detected in biological samples. Recent studies have shown that IL-15 remains inside the cell and can only be secreted outside when it forms a complex with its only receptor, IL-15Rα.

Standard Curve

1. Calculate the mean OD450 value (using the original OD450 value or the corrected OD450 value) of the duplicate readings for each standard, control, and sample. Then, obtain the value of calculation by subtracting the OD450 blank.
2. Create a four parameter logistic curve by plotting the mean absorbance for each standard on the y-axis against the concentration on the x-axis. (Remove the OD450 blank during plotting.) Alternatively, you can use the curve fitting software offered by the microplate reader (e.g. Thermo SkanIt RE software, Curve Expert 1.3 or 1.4).
3. Calculate the sample concentration by substituting OD450 value into the standard curve. Diluted samples should be multiplied by the relevant dilution ratio.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Understanding FRET as a Research Tool for Cellular Studies

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Authors: Shrestha, Dilip; Jenei, Attila; Nagy, Peter; Vereb, Gyoergy; Szoellosi, Janos

Abstract

Communication of molecular species through dynamic association and/or dissociation at various cellular sites governs biological functions. Understanding these physiological processes require delineation of molecular events occurring at the level of individual complexes in a living cell. Among the few non-invasive approaches with nanometer resolution are methods based on Forster Resonance Energy Transfer (FRET). FRET is effective at a distance of 1-10 nm which is equivalent to the size of macromolecules, thus providing an unprecedented level of detail on molecular interactions. The emergence of fluorescent proteins and SNAP- and CLIP- tag proteins provided FRET with the capability to monitor changes in a molecular complex in real-time making it possible to establish the functional significance of the studied molecules in a native environment. Now, FRET is widely used in biological sciences, including the field of proteomics, signal transduction, diagnostics and drug development to address questions almost unimaginable with biochemical methods and conventional microscopies. However, the underlying physics of FRET often scares biologists. Therefore, in this review, our goal is to introduce FRET to non-physicists in a lucid manner. We will also discuss our contributions to various FRET methodologies based on microscopy and flow cytometry, while describing its application for determining the molecular heterogeneity of the plasma membrane in various cell types.

Monocytes differentiated with IL-15 support Th17 and Th1 responses to wheat gliadin: Implications for celiac disease

CLINICAL IMMUNOLOGY

Authors: Harris, Kristina M.; Fasano, Alessio; Mann, Dean L.

Abstract

Interleukin (IL)-15 contributes to the immunopathogenesis of Celiac disease (CD). However, it is not clear how IL-15 affects APC that shape adaptive immune responses to the dietary antigen, gliadin. Using PBMC from healthy individuals, we show that monocytes differentiated with IL-15 (IL15-DC) produced IL-1 beta, IL-6, IL-15, IL-23, TNF alpha and CCL20 in response to pepsin-trypsin digested gliadin (PTG) and activated contact-dependent Th17 and Th1 responses from autologous CD4(+) T cells. Lower concentrations of IL-15 augmented IFN gamma responses to PTG in PBMC from CD patients compared to controls. Thus, IL-15 supports Th17 and Th1 responses to a dietary antigen that is normally well-tolerated in healthy individuals by generating IL15-DC These potentially pathogenic immune responses may result in CD patients and not healthy individuals as a consequence of IL-15 hypersensitivity. Therefore, genetic and/or environmental factors that control IL-15 expression and responsiveness in the intestine likely participate in the pathogenesis of CD. (C) 2010 Elsevier Inc. All rights reserved.