Human Copeptin ELISA Kit

Species Reactivity

Human

Detection Method

Competitive ELISA

Intended Use

This Enzyme Immunoassay kit is designed to detect a specifi peptide and its related peptides based on the principle of "competitive" enzyme immunoassay.

Contents of Kit

1. 20x assay buffer concentrate (50ml).
2. 96 well immunoplate (1 plate).
3. Acetate plate sealer (APS), (3 pieces).
4. Primary antiserum (rabbit anti-peptide IgG) (1 vial).
5. Standard peptide (1 vial).
6. Biotinylated peptide (1 vial).
7. Streptavidin-horseradish peroxidase (SA-HRP) (30μl).
8. Positive control (2 vials).
9. Substrate solution (TMB) (12ml).
10. 2N HCl (15ml).
11. Assay diagram (1 sheet).
12. General protocol (1 book).

Storage

Store the kit at 4°C upon receipt.

Sensitivity

0.12 ng/mL

General Description

The immunoplate in this kit is pre-coated with secondary antibody and the nonspecifi binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate solution. The intensity of the yellow is directly proportional to the amount of biotinylated peptideSA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.

Standard Curve