Human Colony Stimulating Factor 1 Receptor, CSF1R ELISA Kit

Species Reactivity

Human

Detection Method

Sandwich ELISA

Intended Use

The human CSF1R ELISA kit is for the quantitative determination of human CSF1R.
This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on five 96-well plates.

Contents of Kit

1. Capture Antibody: 0.5 mg/mL of mouse anti-CSF1R monoclonal antibody. Dilute to a working concentration of 1 μg/mL in CBS before coating.
2. Detection Antibody: Each vial contains 60 μg biotinylated rabbit anti-CSF1R polyclonal antibody. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C to -70°C. Dilute to a working concentration of 1 μg/mL in detection antibody dilution buffer before use.
3. Standard: Each vial contains 18ng of recombinant CSF1R. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C to -70°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1, 000 pg/mL is recommended.
4. Streptavidin-HRP: 50 μL of streptavidin conjugated to horse-radish-peroxidase. 1:2,000 Dilution in detection antibody dilution buffer before use.

Storage

Keep streptavidin-HRP at 4°C and protect it from prolonged exposure to light. Aliquot all other reagents and store at -20°C to -70°C in a manual defrost freezer.

Sensitivity

15.6 pg/mL

Reconstitution And Storage

Bring all reagents to room temperature before use.
##Capture Antibody: ##0.5 mg/ml of mouse anti-CSF1R monoclonal antibody. Dilute to a working concentration of 1 μg/ml in CBS before coating.
##Detection Antibody: ##Each vial contains 60ug biotinylated rabbit anti-CSF1R polyclonal antibody. Reconstitute with 1ml detection antibody dilution buffer. After reconstitution, store at -20°C to -70°C. Dilute to a working concentration of 1 μg/ml in detection antibody dilution buffer before use.
##Standard: ##Each vial contains 18ng of recombinant CSF1R. Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20°C to -70°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1, 000 pg/ml is recommended.
##Streptavidin-HRP: ##50μl of streptavidin conjugated to horse-radish-peroxidase. 1: 2, 000 Dilution in detection antibody dilution buffer before use.