Sample
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Intended Use
For the quantitation of Human CEACAM5 concentrations in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Contents of Kit
1.1.Anti-Human CEACAM5 Pre-coated 96-well Strip Microplate, 1, 12 strips of 8 wells, Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 4°C provided this is within the expiration date of the kit.
2.Human CEACAM5 Standard, 2, 20 ng/tube, Discard the CEACAM5 stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours.
3.Human CEACAM5 Biotinylated Antibody (100x), 1, 100 μl
4.Avidin-Biotin-Peroxidase Complex (100x), 1, 100 μl
5.Sample Diluent, 1, 30 ml
6.Antibody Diluent, 1, 12 ml
7.Avidin-Biotin-Peroxidase Diluent, 1, 12 ml
8.Color Developing Reagent (TMB), 1, 10 ml
9.Stop Solution 1, 10 ml
10.Wash Buffer (25x), 1, 20 ml
11.Plate Sealers, 4 Piece
3-10 May be stored for up to 1 month at 4°C provided this is within the expiration date of the kit.
Storage
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Precision
CD spends great efforts in documenting lot-to-lot variability and ensuring our assay kits produce robust data that are reproducible.
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Detection Range
312 pg/mL - 20,000 pg/mL
General Description
Carcinoembryonic antigen (CEA) is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60% carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.
Standard Curve
The highest O.D. value might be higher or lower than in the example. The experiment result is statistically significant if the highest O.D. value is no less than 1.0.
A standard curve is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Citations
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COA
Certificate of Analysis
