Human Aspergillus Fumigatus IgG ELISA Kit

Sample

Serum, plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

The Aspergillus fumigatus IgG is quantitative and qualitative test for the detection of human antibodies in serum or plasma against Aspergillus fumigatus. For Research Use Only. Not for use in diagnostic procedures.

Contents of Kit

1. Break apart microtiter test strips each with 8 antigen coated single wells (altogether 96) MTP, 1 frame, the coating material is inactivated, 12
2. Standard serum (ready-to-use) STD Human serum in phosphate buffer with protein; negative for anti-HIVAb, HBs-Ag (Hepatitis B-Virus-surface antigen) and anti-HCV-Ab; preservative: < 0.1 % sodium azide, colouring: Amaranth O, 2 × 2 ml
3. Negative control serum (ready-to-use) NEG Human serum in phosphate buffer with protein; negative for anti-HIV-Ab, HBs-Ag (Hepatitis B-Virus-surface antigen) and anti-HCV; preservative: < 0.1 % sodium azide, colouring: Lissamin green V, 2 ml
4. Anti-human-IgG-conjugate (ready-to-use) APC Anti-human-IgG from goat (polyclonal), conjugated to alkaline phosphatase, stabilized with protein stabilization solution, preservative: 0.01 % methylisothiazolone, 0.01 % bromnitrodioxane, 13 ml
5. Washing solution concentrate (sufficient for 1 litre) WASH Sodium chloride solution with Tween 20, 30 mM Tris, preservative: < 0.1 % sodium azide, 33.3 ml
6. Dilution buffer DILB Phosphate buffer with protein and Tween 20; preservative: < 0.1 % sodium azide 0.01 g/l Bromphenol blue sodium salt, 2 × 50 ml
7. Stopping solution STOP 1.2 N sodium hydroxide, 15 ml
8. Substrate (ready-to-use) pNPP Para-nitrophenylphosphate, solvent free buffer, preservative: < 0.1 % sodium azide, (Substrate in unopened bottle may have a slightly yellow coloring. This does not reduce the quality of the product!), 13 ml

Storage

1. Microtiter strips (antigen). After opening at 2-8°C in closed aluminum bag with desiccant. Strips which are not used must be stored in the press-seal bag of aluminum compound foil under dry and airtight conditions! 4 weeks.
2. Control sera / standard sera. After opening at 2-8°C. Until expiry date; 24 months from date of production.
3. Conjugate ready-to-use solution, at 2-8°C. Avoid contamination (sterile tips!). Until expiry date 28 months from date of production
4. Dilution buffer. After opening at 2-8°C, 24 months. Discard cloudy solutions! Unopened, until expiry date; 36 months from date of production.
5. Washing solution. Concentrate after opening at 2-8°C, until expiry date. Working dilution at 2-8°C, 2 weeks. Working dilution at room temperature, 1 week. Bottles used for the working dilution should be cleaned regularly, discard cloudy solutions.
6. Substrate, ready-to-use solution at 2-8°C, protected from light! Until expiry date 24 months from date of production. Avoid contamination (sterile tips!) Discard when solution turns yellow (extinction against distilled water. > 0.25).
7. Stopping solution, after opening at room temperature, until expiry date.

General Description

The fungus Aspergillus belongs to the group of ascomycetes. These organisms develop branched mycelia. They spread through conidiospores which are released from mycelia as extremely resistant lasting spores. 20 species of Aspergillus are human pathogens being opportunistic infectious agents. The most important species is Aspergillus fumigatus.
Aspergillus fumigatus can cause multiple allergic and invasive diseases. The pathogenesis of an invasive Aspergillus infection takes place in several steps: The fungal spores are transmitted through the air as a so called "bioaerosol" and are absorbed by the lungs. Due to their size of about 3 μm they reach the lung alveoli. In case of an invasive aspergillosis fungus hyphae penetrate the bronchial mucosa and the surrounding lung parenchyma with the aid of released proteinases. The Aspergillus spores tend to penetrate the blood vessels and settle down in remote tissues after a hematogen dissemination.