Sample
nuclear, cell lysates
Species Reactivity
Human, Mouse, Rat
Detection Method
Indirect ELISA
Contents of Kit
1. 12x 8-Well dsDNA Oligonucleotide Coated Microstrips
2. 100x Anti-Phospho Target Primary Antibody
3. 100x Anti-Target Primary Antibody
4. HRP-Conjugated Anti-Rabbit IgG Secondary Antibody
5. Nuclear Lysate Positive Control
6. Wild-Type Consensus dsDNA Oligonucleotide
7. Mutant Consensus dsDNA Oligonucleotide
8. 10x Wash Buffer
9. 2x Binding Buffer
10. Primary Antibody Diluent
11. 100x Protease and Phosphatase Inhibitors
12. Nuclear Wash Buffer
13. Cytoplasmic Extraction Buffer
14. Nuclear Extraction Buffer
15. Ready-to-Use Substrate
16. Stop Solution
17. Adhesive Plate Seals
18. Technical Manual
Citations
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Rubio-Cabezas, O; Edghill, EL; et al. Testing for monogenic diabetes among children and adolescents with antibody-negative clinically defined Type 1 diabetes. DIABETIC MEDICINE 26:1070-1074(2009).
Huang, JM; Karakucuk, V; et al. Expression of HNF4 alpha variants in pancreatic islets and Ins-1 beta cells. DIABETES-METABOLISM RESEARCH AND REVIEWS 24:533-543(2008).