Hepcidin 25 (bioactive) HS ELISA Kit (DEIA-XY2273)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Species Reactivity
Intended Use
The Hepcidin 25 (bioactive) HS ELISA Kit high sensitive enzyme immunoassay for measurement of Hepcidin-25 in serum and plasma.
Contents of Kit
1. Microtiterwells, 12 x 8 (break apart) strips, 96 wells;
2. Zero Standard, 1 vial, 3 mL
3. Standard (Standard 1 - 5), 5 vials, 0.5 mL
4. Control Low & High, 2 vials, 0.5 mL each
5. Enzyme Conjugate, 1 vial, 7 mL
6. Enzyme Complex, 1 vial, 14 mL
7. Substrate Solution, 1 vial, 14 mL
8. Stop Solution, 1 vial, 14 mL
9. Wash Solution, 1 vial, 30 mL (40x concentrated)
2°C to 8°C
Performance Characteristics
Assay Dynamic Range
The range of the assay is between 0.153 ng/mL – 81 ng/mL.

Specificity of Antibodies (Cross Reactivity)
The following substances were tested for cross reactivity of the assay:
Standard Curve
Standard Optical Units (450 nm)
Standard 0 (0 ng/mL) 2.03
Standard 1 (1 ng/mL) 1.70
Standard 2 (3 ng/mL) 1.29
Standard 3 (9 ng/mL) 0.77
Standard 4 (27 ng/mL) 0.37
Standard 5 (81 ng/mL) 0.17
Reconstitution And Storage
When stored at 2 to 8°C unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date. Opened reagents must be stored at 2 to 8°C. Microtiter wells must be stored at 2 to 8°C. Once the foil bag has been opened, care should be taken to close it tightly again. Opened kits retain activity for two months if stored as described above.


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