Florfenicol Amine [HRP]

The prevalence of multi-drug resistant S. pseudintermedius in Israel (IMDR), as assessed by a standardized disc-diffusion method, increased between 2004 and 2012 and decreased subsequently. Isolates were considered as IMDR if they were resistant to penicillin, oxacillin, ampicillin, amoxicillin-clavulanate, cephalothin, clindamycin, enrofloxacin, sulfamethoxazole-trimethoprim, gentamicin, erythromycin and tetracycline, or susceptible to one-tetracycline or sulfamethoxazole-trimethoprim. Isolates resistant to oxacillin were considered resistant to all beta-lactam antibiotics. The susceptibility to chloramphenicol and florfenicol was tested for 61 IMDR isolates of S. pseudintermedius to evaluate if phenicols may be considered as treatment options. Among them, 43 isolates were resistant to chloramphenicol. Inhibition zones for florfenicol (for which no interpretation standards for canine or feline isolates exist) were between 22 and 28 mm and between 18 and 21 mm for 59 and 2 isolates, respectively. IMDR still represents a challenge for veterinary medicine in Israel requiring further improvement of the standard hygienic procedures in veterinary settings as well as the search for alternative antimicrobial treatments. Puppies imported from Thailand may be a potential source of IMDR strains.

A simple and straightforward technique for coating microplate wells with molecularly imprinted polymer nanoparticles (nanoMIPs) to develop assays similar to the enzyme-linked immunosorbent (ELISA) assay to determine and quantify florfenicol (FF) in real food samples such as liquid milk and salmon muscle is presented here. The nanoMIPs were synthesized by a solid-phase approach with an immobilized FF (template) and characterized using dynamic light scattering, a SPR-2 biosensor system and transmission electron microscopy. Immobilization of nanoMIPs was conducted by preparing a homogenous solution of FF-nanoMIPs in water mixed with polyvinyl alcohol (PVA) 0.2% (w/v) in each well of a microplate. The detection of florfenicol was achieved in competitive binding experiments with a horseradish peroxidase florfenicol (FF-HRP) conjugate. The assay made it possible to measure FF in buffer and in real samples (liquid milk and salmon muscle) within the range of 60 80 and 90-100 ng/mL, respectively. The immobilized nanoMIPs were stored for six weeks at room temperature and at 5 degrees C. The results indicate good signal recovery for all FF concentrations in spiked milk samples, without any detrimental effects to their binding properties. The high affinity of nanoMIPs and the lack of a requirement for cold chain logistics make them an attractive alternative to traditional antibodies used in ELISA.