Product Overview
HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The FOLR1 knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
Alternative Names
FOLR1; folate receptor 1 (adult); FBP; FOLR; folate receptor alpha; FR-alpha; KB cells FBP; folate binding protein; folate receptor, adult; adult folate-binding protein; ovarian tumor-associated antigen MOv18;
Application Notes
Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Dilution
Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
Concentration
The protein concentration was determined with BCA assay.
Storage
Store at -20°C. Avoid repeated freeze-thaw cycles.
Citations
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Rajagopalan, R; Poreddy, AR; et al. Folate receptor targeted Type 1 photosensitizer bioconjugates for tumor visualization and phototherapy. OPTICAL METHODS FOR TUMOR TREATMENT AND DETECTION: MECHANISMS AND TECHNIQUES IN PHOTODYNAMIC THERAPY XX 7886:-(2011).
Thomas, A; Maltzman, J; et al. Farletuzumab in lung cancer. LUNG CANCER 80:15-18(2013).
COA
Certificate of Analysis
