Detection Method
Indirect ELISA
Intended Use
The Coagulation Factor III Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Coagulation Factor III protein expression profile in cells. The kit can be used for measuring the relative amounts of Coagulation Factor III in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Coagulation Factor III.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 1 plate
2. 10x TBS: 24 mL (10x), Clear
3. Quenching Buffer: 24 mL (1x), Clear
4. Blocking Buffer: 50 mL (1x), Clear
5. 10x Wash Buffer: 50 mL (10x), Clear
6. 100x Anti-Coagulation Factor III Antibody (Rabbit Polyclonal): 60 μL (100x), Purple
7. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green
8. HRP-Conjugated Anti-Rabbit IgG Antibody: 6 mL (1x), Glass
9. HRP-Conjugated Anti-Mouse IgG Antibody: 6 mL (1x), Glass
10. Primary Antibody Diluent: 12 mL (1x), Clear
11. Ready-to-Use Substrate: 12 mL (1x), Brown
12. Stop Solution: 12 mL (1x), Clear
13. Crystal Violet Solution: 6 mL (1x), Glass
14. SDS Solution: 24 mL (1x), Clear
15. Adhesive Plate Seals: 4 seals
Citations
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Jenh, CH; Cox, MA; et al. Human B cell-attracting chemokine 1 (BCA-1; CXCL13) is an agonist for the human CXCR3 receptor. CYTOKINE 15:113-121(2001).
Bruenke, J; Fischer, B; et al. A recombinant bispecific single-chain Fv antibody against HLA class II and Fc gamma RIII (CD16) triggers effective lysis of lymphoma cells. BRITISH JOURNAL OF HAEMATOLOGY 125:167-179(2004).