Sample
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Detection Method
Sandwich ELISA
Intended Use
Rat Phosphorylation Extracellular signal Regulated Kinase 1/2 ELISA Kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of the Phosphorylation Extracellular signal Regulated Kinase 1/2. This ELISA kit is for research use only, not for therapeutic or diagnostic applications.
Contents of Kit
1. MICROTITER PLATE: 96 wells
2. ENZYME CONJUGATE: 6.0 mL or 10 ml
3. STANDARD A-F: 1 vial each
4. SUBSTRATE A: 6 mL
5. SUBSTRATE B: 6 mL
6. STOP SOLUTION: 6 mL
7. WASH SOLUTION (100 x): 10 mL
8. BALANCE SOLUTION: 3 mL
Storage
All components of this kit are stable at 2-8°C until the kit's expiration date.
Detection Range
10-250 ng/mL
Citations
Publication ()
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Stimulation of atypical cannabinoid receptor GPR55 abolishes the symptoms of detrusor overactivity in spontaneously hypertensive rats
Andrzej Wróbel, Anna Serefko, Aleksandra Szopa, Ewa Poleszak
Applications: ELISA
Reactive species: Rat
"Abstract: Overactive bladder is a troublesome disease that affects 15% of the population in developed countries. Since pharmacotherapy of this condition is frequently associated with side effects, the better tolerated drugs are being searched for. The main objective of our study was to check whether activation of the atypical cannabinoid receptor GPR55 would normalize the changes in cystometric, cardiovascular and biochemical parameters in the hypertensive female Wistar-Kyoto rats presenting the symptoms of overactive bladder accompanied by inflammation and oxidative damage in the urinary tracts. A 14-day intra-arterial administration of O-1602 (0.25 mg/kg/day), a potent agonist of GRP55 receptors, was able to abolish the signs of detrusor overactivity, inflammation and oxidative damage in the urinary bladder of the spontaneously hypertensive animals. Moreover, it increased their heart rate, reduced the mean blood pressure, and normalized the levels of several proteins that play a significant role in the proper functioning of the urinary bladder (i.e., calcitonin gene related peptide, organic cation transporter 3, extracellular signal-regulated kinase 1/2, vesicular acetylcholine transporter, RhoA). Based on the outcomes of our experiments, the atypical cannabinoid receptor GPR55 has emerged as a potential drug target for the treatment of overactive bladder in female subjects. It could be particularly attractive in the cases in which this condition is accompanied with elevated blood pressure, though further studies on this subject are needed."
Article snippet: Levels of following markers were determined in the bladder urothelium: calcitonin gene related peptide (*), malondialdehyde (MAL; *), 3-nitrotyrosine (NIT; *), organic cation transporter 3 (OCT3; *), and extracellular signal-regulated kinase 1/2 (ERK1/2; Creative Diagnostics, CN DEIA-BJ2227). 
Figure 1. Influence of the 14-day intra-arterial administration of O-1602 (0.25 mg/kg/day) on levels of ERK1/2 in the bladder urothelium in the bladder detrusor muscle in wild-type and spontaneously hypertensive female rats
COA
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