Intended Use
The EGR1/2 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor EGR1/2 protein expression profile in cells. The kit can be used for measuring the relative amounts of EGR1/2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on EGR1/2.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate, 1 Plate
2. 10× TBS, 24 ml (10×), Stability after first use: 1 month at 4°C
3. Quenching Buffer, 24 ml (1×), Stability after first use: 1 month at 4°C
4. Blocking Buffer, 50 ml (1×), Stability after first use: 1 month at 4°C
5. 15× Wash Buffer, 50 ml (15×), Stability after first use: 1 month at 4°C
6. 100× Anti- EGR1/2 Antibody, 60 μl (100×), Stability after first use: 1 month at 4°C
7. 100× Anti-GAPDH Antibody, 60 μl (100×), Stability after first use: 1 month at 4°C
8. HRP-Conjugated Anti-Rabbit IgG Antibody, 6 ml (1×), Stability after first use: 1 month at 4°C
9. HRP-Conjugated Anti-Mouse IgG Antibody, 6 ml (1×), Stability after first use: 1 month at 4°C
10. Primary Antibody Diluent, 12 ml (1×), Stability after first use: 1 month at 4°C
11. Ready to Use Substrate, 12 ml (1×), Stability after first use: 1 month at 4°C
12. Stop Solution, 12 ml (1×), Stability after first use: 1 month at 4°C
13. Crystal Violet Solution 6 ml (1×), Stability after first use: 1 month at 4°C
14. SDS Solution, 24 ml (1×), Stability after first use: 1 month at 4°C
15. Adhesive Plate Seals, 2 Seals, Stability after first use: 1 month at 4°C
COA
Publication 
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