This kit is intended for the quantitative detection of residual E. coli host cell proteins in intermediates, in-process materials, and final products of biopharmaceuticals and pharmaceuticals. The product has been validated for use with most E. coli engineered cell lines (e.g., DH5α, BL21, TOP10). For research use only. MUST NOT be used for clinical diagnosis.
Contents of Kit
No.
Components
Size
Storage Conditions
1
E.coli HCP Coated Plate
8 wells × 12 strips
2-8 °C
2
E.coli HCP Standard
1 mL × 8
-20 °C
3
100× Anti-E.coli HCP-HRP
200 μL × 1
-20 °C
4
Sample Diluent
15 mL × 2
2-8 °C
5
20× Wash Buffer
25 mL × 2
2-8 °C
6
Stop Solution
10 mL × 1
2-8 °C
7
TMB Substrate A
6 mL×1
2-8 °C
8
TMB Substrate B
6 mL×1
2-8 °C
9
Sealer film
5 films
Storage
Unopened kits retain stability for 12 months when stored at -20°C.
Precision
CV%≤10%, RE%≤ ±15%
Detection Range
3.125-200ng/mL
Sensitivity
Limit of Quantitation: 1ng/mL Limit of Detection: 0.3ng/mL
Standard Curve
Citations
Publication ()
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