The E.coli Expression Strain HCP ELISA kit is designed for quantifying host cell proteins (HCPs) in Escherichia coli expression strain BL21. It is suitable for quantifying HCPs in intermediate and crude samples of recombinant protein products, including interleukins (IL), interferons (rhIFN), granulocyte-macrophage colony-stimulating factors (rhGM-CSF), necrosis factors (rhTNF), growth factors (EGF/FGF/PDGF), etc.
Contents of Kit
No.
Components
Size
Storage Conditions
1
E.coli HCP-E Standard
2 vials
2-8°C
2
Pre-coated microtitre strips
1 × 12 × 8
2-8°C
3
Standard Dilution
1 × 1.5 ml
2-8°C
4
Samples/Antibody Dilution Buffer
2 × 25 ml
2-8°C
5
Washing Buffer (10× conc.)
2 × 25 ml
2-8°C
6
Detection Antibody-HRP (100× conc.)
1 × 120 μl
2-8°C
7
TMB Substrate
12 ml
2-8°C
8
Stop Solution
6 ml
2-8°C
Storage
Store the unopened reagents at 2–8°C until expiration date. Do not use components beyond the expiry date indicated on the kit labels. Once opened the reagents are stable for 2 months when stored at 2–8°C. The dissolved standard can be stable for 30 days at 2-8°C. Once the resealable pouch has been opened, care should be taken to close it tightly again.
Detection Range
1.5-243 ng/ml
Detection Limit
1.5 ng/ml
Citations
Publication ()
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