Detection Method
Competitive ELISA
Intended Use
The DHEA ELISA is an enzyme immunoassay for the quantitative in vitro diagnostic measurement of Dehydroepiandrosterone (DHEA) in serum.
Contents of Kit
1. Microtiter wells, 12 x 8 (break apart) strips, 96 wells
2. Standard (Standard 0-5), 6 vials, 1 mL
3. Enzyme Conjugate, 1 vial, 14 mL
4. Substrate Solution, 1 vial, 14 mL
5. Stop Solution, 1 vial, 14 mL
6. Wash Solution, 1 vial, 30 mL (40X concentrated).
Storage
Store the kit at 2-8°C. Keep microwells sealed in a dry bag with desiccants. For more detailed information, please download the following document on our website.
Detection Range
0-30 ng/mL.
Citations
Publication ()
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The mitochondria-targeted antioxidant SkQ1 but not N-acetylcysteine reverses aging-related biomarkers in rats
Kolosova NG, Stefanova NA, Muraleva NA, Skulachev VP
Applications: ELISA
Reactive species: Rat
"Abstract: Although antioxidants have been repeatedly tested in animal models and clinical studies, there is no evidence that antioxidants reduce already developed age-related decline. Recently we demonstrated that mitochondria targeted antioxidant 10-(6'-plastoquinonyl) decyltriphenylphosphonium (SkQ1) delayed some manifestations of aging.Here we compared effects of SkQ1 and N-acetyl-L-cysteine (NAC) on age-dependent decline in blood levels of leukocytes,growth hormone (GH), insulin-like growth factor-1 (IGF-1), testosterone, dehydroepiandrosterone (DHEA) in Wistar and senescence-accelerated OXYS rats. When started late in life, supplementation with SkQ1 not only prevented age-related decline but also significantly reversed it. With NAC, all the observed effects were of the lower magnitude compared with SkQ1 (in spite of that dose of NAC was 16000 times higher). We suggest that supplementation with low doses of SkQ1 is a promising intervention to achieve a healthy ageing."
Article snippet: The levels of GH, IGF-1, testosterone and DHEA-S in the blood serum were analyzed before and after treatment with SkQ1 or NAC in OXYS and Wistar rats (two times total for each rat) and compared with intact 3-month-old rats (n=10) of the same strains. GH, DHEA were measured by ELISA (Creative Diagnostics, USA). 
Figure 1. Levels of GH, IGF-1, DHEA-S and testosterone (ng/ml) in serum of intact Wistar and OXYS rats at the age of 3, 19 and 23 months and of rats treated with SkQ1 or NAC from the age of 19 months to 23 months.
COA
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