Human NLRP3 blocking peptide

Name: Human NLRP3 blocking peptide
SKU: CDBP0889
Rating: 5 (1 reviews)

Synthetic Human NLRP3 blocking peptide for Apuri, BL, ELISA

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Product Overview

Blocking/Immunizing peptide for anti-Cryopirin/NALP3/NLRP3 antibody

Target

Cryopirin / NALP3 / NLRP3

Nature

Synthetic

Tag/Conjugate

Unconjugated

Application Notes

For in vitro research use only. Not intended for any diagnostic or therapeutic purpose. Not for human or animal consumption.

Format

Lyophilized powder

Preservative

None

Storage

Shipped at ambient temperature, store at -20°C.

Gene ID

114548

UniProt ID

Q96P20

Antigen Description

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq, Oct 2008]

Function

ATP binding; peptidoglycan binding; protein binding;

Synonyms

NLRP3; NLR family, pyrin domain containing 3; AII; AVP; FCU; MWS; FCAS; CIAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL; NACHT, LRR and PYD domains-containing protein 3; cryopyrin; caterpiller protein 1.1; PYRIN-containing APAF1-like protein 1; NACHT, LRR and PYD containing protein 3; cold autoinflammatory syndrome 1 protein; NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3;

Citations

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Activated NLR family pyrin domain containing 3 (NLRP3) inflammasome in keratinocytes promotes cutaneous T-cell response in patients with vitiligo (vol 145, pg 632, 2020)

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

Authors: [Anonymous]

Abstract

Metabolic Regulation of Inflammasome Activity Controls Embryonic Hematopoietic Stem and Progenitor Cell Production

DEVELOPMENTAL CELL

Authors: Frame, Jenna M.; Kubaczka, Caroline; Long, Timothy L.; Esain, Virginie; Soto, Rebecca A.; Hachimi, Mariam; Jing, Ran; Shwartz, Arkadi; Goessling, Wolfram; Daley, George Q.; North, Trista E.

Abstract

Embryonic hematopoietic stem and progenitor cells (HSPCs) robustly proliferate while maintaining multilineage potential in vivo; however, an incomplete understanding of spatiotemporal cues governing their generation has impeded robust production from human induced pluripotent stem cells (iPSCs) in vitro. Using the zebrafish model, we demonstrate that NLRP3 inflammasome-mediated interleukin-1-beta (IL1 beta) signaling drives HSPC production in response to metabolic activity. Genetic induction of active IL1 beta or pharmacologic inflammasome stimulation increased HSPC number as assessed by in situ hybridization for runxl/cmyb and flow cytometry. Loss of inflammasome components, including il1b, reduced CD41(+) HSPCs and prevented their expansion in response to metabolic cues. Cell ablation studies indicated that macrophages were essential for initial inflammasome stimulation of il1rl1 (+) HSPCs. Significantly, in human iPSC-derived hemogenic precursors, transient inflammasome stimulation increased multilineage hematopoietic colony-forming units and T cell progenitors. This work establishes the inflammasome as a conserved metabolic sensor that expands HSPC production in vivo and in vitro.