Coxiella burnetii ( Q-Fever ) Phase 1 IgG ELISA Kit

Sample

serum and plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

CD ELISA Coxiella burnetii Phase 1 IgG is qualitative tests for detection of human antibodies in serum and plasma directed against Coxiella burnetii Phase 1. For Research Use Only. Not for use in diagnostic procedures.

Storage

1. Microtiter strips (antigen), after opening at 2-8°C in closed aluminum bag with desiccant. 4 weeks. Strips which are not used must be stored in the press-seal bag of aluminum compound foil under dry and airtight conditions!
2. Control sera, after opening at 2-8°C until expiry date; 24 months from date of production.
3. Conjugate, ready-to-use solution, at 2-8°C. Avoid contamination (sterile tips!). until expiry date 28 months from date of production.
4. Dilution buffer, after opening at 2-8°C, 24 months. Discard cloudy solutions! Unopened: until expiry date; 36 months from date of production.
5. Washing solution, concentrate after opening at 2-8°C (until expiry date), working dilution at 2-8°C (2 weeks), working dilution at room temperature(1 week).
6. Substrate, ready-to-use solution at 2-8°C, protected from light! Avoid contamination (sterile tips!) Discard when solution turns yellow (extinction against distilled water > 0.25). Until expiry date 24 months from date of production.
7. Stopping solution, after opening at room temperature, until expiry date

General Description

The Q-fever zoonosis is a globally ubiquitous disease. Its pathogen is the Rickettsia Coxiella burnetii, a small, gram-negative, obligate intracellular bacterium. Coxiella burnetii multiplies in the digestive system of ticks (Dermacentor marginatus in Germany) and in the trophoblasts of a host placenta. Accordingly, tick faeces and the afterbirth of infected mammals (predominantly sheep) are highly infectious. Therefore, occupational groups having direct contact with farm animals are at great risk of exposure. Infection usually results from inhalation of contaminated aerosols, especially during the dry summer months. The incubation time for Q-fever is approximately 2-4 weeks following exposure. Influenzalike symptoms are often observed. Diagnosis of Q-fever is currently based on serologic techniques. The use of ELISA test systems is recommended by the WHO because of high sensitivities and specificities and the ability to perform a differential analysis of the antibody response.