Sample
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Detection Method
Sandwich ELISA
Intended Use
Human Collagen Type VII ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of the Collagen Type VII. This ELISA kit is for research use only, not for therapeutic or diagnostic applications.
Contents of Kit
1. MICROTITER PLATE: 96 wells
2. ENZYME CONJUGATE: 6.0 mL or 10 ml
3. STANDARD A-F: 1 vial each
4. SUBSTRATE A: 6 mL
5. SUBSTRATE B: 6 mL
6. STOP SOLUTION: 6 mL
7. WASH SOLUTION (100 x): 10 mL
8. BALANCE SOLUTION: 3 mL
Storage
All components of this kit are stable at 2-8°C until the kit's expiration date.
Detection Range
0.5-10 ng/mL
Citations
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Hovnanian, A; Rochat, A; et al. Characterization of 18 new mutations in COL7A1 in recessive dystrophic epidermolysis bullosa provides evidence for distinct molecular mechanisms underlying defective anchoring fibril formation. AMERICAN JOURNAL OF HUMAN GENETICS 61:599-610(1997).
Vindevoghel, L; Kon, A; et al. Smad-dependent transcriptional activation of human type VII collagen gene (COL7A1) promoter by transforming growth factor-beta. JOURNAL OF BIOLOGICAL CHEMISTRY 273:13053-13057(1998).