Clock ELISA Kit

Name: Clock ELISA Kit
SKU: DEIA-XYA435
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

cultured cells

Species Reactivity

Human; Mouse; Rat

Detection Method

Indirect ELISA

Intended Use

The Clock Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Clock protein expression profile in cells. The kit can be used for measuring the relative amounts of Clock in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Clock.

Contents of Kit

1. 96-Well Cell Culture Clear-Bottom Microplate: 1 plate
2. 10x TBS: 24 mL (10x), Clear
3. Quenching Buffer: 24 mL (1x), Clear
4. Blocking Buffer: 50 mL (1x), Clear
5. 10x Wash Buffer: 50 mL (10x), Clear
6. 100x Anti-Clock Antibody (Rabbit Polyclonal): 60 μL (100x), Purple
7. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green
8. HRP-Conjugated Anti-Rabbit IgG Antibody: 6 mL (1x), Glass
9. HRP-Conjugated Anti-Mouse IgG Antibody: 6 mL (1x), Glass
10. Primary Antibody Diluent: 12 mL (1x), Clear
11. Ready-to-Use Substrate: 12 mL (1x), Brown
12. Stop Solution: 12 mL (1x), Clear
13. Crystal Violet Solution: 6 mL (1x), Glass
14. SDS Solution: 24 mL (1x), Clear
15. Adhesive Plate Seals: 4 seals

Storage

4°C/6 Months

Citations

Have you cited DEIA-XYA435 in a publication? Let us know and earn a reward for your research.

Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Stromal Expression of the Core Clock GenePeriod 2Is Essential for Tumor Initiation and Metastatic Colonization

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY

Authors: Shaashua, Lee; Mayer, Shimrit; Lior, Chen; Lavon, Hagar; Novoselsky, Alexander; Scherz-Shouval, Ruth

Abstract

The circadian clock regulates diverse physiological processes by maintaining a 24-h gene expression pattern. Genetic and environmental cues that disrupt normal clock rhythms can lead to cancer, yet the extent to which this effect is controlled by the cancer cells versus non-malignant cells in the tumor microenvironment (TME) is not clear. Here we set out to address this question, by selective manipulation of circadian clock genes in the TME. In two different mouse models of cancer we find that expression of the core clock genePer2in the TME is crucial for tumor initiation and metastatic colonization, whereas another core gene,Per1, is dispensable. We further show that loss ofPer2in the TME leads to significant transcriptional changes in response to cancer cell introduction. These changes may contribute to a tumor-suppressive microenvironment. Thus, our work unravels an unexpected protumorigenic role for the core clock genePer2in the TME, with potential implications for therapeutic dosing strategies and treatment regimens.

Dwell-time based stability analysis and L-2 control of LPV systems with piecewise constant parameters and delay

SYSTEMS & CONTROL LETTERS

Authors: Zakwan, Muhammad; Ahmed, Saeed

Abstract

Dwell-time based stability conditions for a class of LPV systems with piecewise constant parameters under time-varying delay are derived using clock-dependent Lyapunov-Krasovskii functional. Sufficient synthesis conditions for clock-dependent gain-scheduled state-feedback controllers ensuring L-2-performance are also provided. Several numerical and practical examples, to illustrate the efficacy of the results, are given. (C) 2020 Elsevier B.V. All rights reserved.