Clover yellow vein virus-strain C81 (ClYVV-C81) ELISA Kit

The sequence of 1399 nucleotides from the 3' region of the RNA of clover yellow vein potyvirus (ClYVV-C) isolated from Calanthe sp. was determined. The capsid protein cistron was composed of 915 nucleotides, and corresponded to a region encoding 305 amino acids with a calculated Mr of 34 900; the adjacent 3' non-coding region was 74 nucleotides long. The length of the capsid protein gene of ClYVV-C is longer, but the 3' non-coding region is shorter than those of other ClYVV strains (ClYVV-30, ClYVV-NZ and ClYVV-B). The nucleotide sequence of the capsid protein gene and the 3' non-coding region of ClYVV-C RNA showed significantly higher homology with those of other ClYVV strains. These data suggest that the ClYVV-C capsid gene arose from a frame-shift mutation of the capsid gene of another ClYVV strain, or vice versa. ClYVV-C has a closer affinity to other ClYVV strains and to bean yellow mosaic virus than to the other potyviruses.

Degenerate primers were used to amplify virus sequences from imported lilies in Zhejiang province, China. Two viruses, Lily mottle virus (LMoV, genus Potyvirus) and Lily symptomless virus (LSV, genus Carlavirus) were detected, purified and completely sequenced from a mixed infection in a plant raised from bulbs imported from the Netherlands. The sequence of LMoV was 9644 nt long and encoded a polyprotein of 3095 amino acids with a calculated M-r of 351.0 kDa that had only 45.1-54.4% identity to other completely sequenced potyviruses. Phylogenetic analysis of the complete polyproteins of members of the genus demonstrated that LMoV was distantly grouped with LYSV, BYMV and ClYVV. Two partial LMoV sequences from different cultivars were identical to one another and very similar (98.3% identical nucleotides) to the corresponding region of the complete sequence. Analysis of the coat protein sequences of LMoV isolates revealed two subgroups, corresponding to the earlier "Tulip breaking virus lily strain" and "Tulip band breaking virus" isolates. Our newly-determined isolates showed an extremely close relationship to the first of these. The LSV sequence was 8393 nucleotides long and had the typical carlavirus genome organization. The ORF1 protein was most closely related to that of Blueberry scorch virus (57.2% identical amino acids). Sequences of 1796 nt at the 3'-end of three additional LSV isolates from different cultivars were very similar (>98% identical nucleotides) to the corresponding region of the complete sequence. This is the first report of complete sequences for LMoV and LSV.