Clover yellow vein virus-strain C81 & Pratt (ClYVV-C81&Pratt) ELISA Kit

Name: Clover yellow vein virus-strain C81 & Pratt (ClYVV-C81&Pratt) ELISA Kit
SKU: DEIAPV130
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

host plants

Intended Use

The test can be used to detect ClYVV-strain C81 and strain Pratt and other all other isolates in infected host plants.

Storage

All reagent components should be stored at the recommended temperature to assure their full shelf life. The kit should be used within six months of purchase.

Sensitivity

Sensitivity of the ELISA is relatively high. The virus can be consistently detected in infected plant tissues diluted at 1:810.

General Description

Clover yellow vein virus (ClYVV) is a plant pathogenic virus in the genus Potyvirus and the virus family Potyviridae. Like other members of the Potyvirus genus, ClYVV is a monopartite strand of positive-sense, single-stranded RNA surrounded by a capsid made for a single viral encoded protein. The virus is a filamentous particle that measures about 760 nm in length. This virus is transmitted by several species of aphids in a nonpersistent manner and by mechanical inoculation.
Clover yellow vein virus, first named in 1965, was isolated from white clover (Trifolium repens) in a garden in England. A survey of English pastures found it to be widespread throughout England. In 1969, a similar virus was isolated in Canada and it was speculated at the time that the virus was also common in the United States but had been misidentified as a strain of Bean yellow mosaic virus (BYMV). With time, using serological, host range studies and eventually sequence data, this proved to be true. For example, several species of Commelinaceae, reported to be infected by BYMV, were found susceptible to ClYVV instead. ClYVV and BYMV are now considered to be different potyviruses, both of which infect many members of the Fabaceae (or Leguminosae) worldwide.

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Documents

pdf COA

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QA & Reviews

Customer Reviews

References

Occurrence and sequences of Lily mottle virus and Lily symptomless virus in plants grown from imported bulbs in Zhejiang province, China

ARCHIVES OF VIROLOGY

Authors: Zheng, HY; Chen, J; Zhao, MF; Lin, L; Chen, JP; Antoniw, JF; Adams, MJ

Abstract

Degenerate primers were used to amplify virus sequences from imported lilies in Zhejiang province, China. Two viruses, Lily mottle virus (LMoV, genus Potyvirus) and Lily symptomless virus (LSV, genus Carlavirus) were detected, purified and completely sequenced from a mixed infection in a plant raised from bulbs imported from the Netherlands. The sequence of LMoV was 9644 nt long and encoded a polyprotein of 3095 amino acids with a calculated M-r of 351.0 kDa that had only 45.1-54.4% identity to other completely sequenced potyviruses. Phylogenetic analysis of the complete polyproteins of members of the genus demonstrated that LMoV was distantly grouped with LYSV, BYMV and ClYVV. Two partial LMoV sequences from different cultivars were identical to one another and very similar (98.3% identical nucleotides) to the corresponding region of the complete sequence. Analysis of the coat protein sequences of LMoV isolates revealed two subgroups, corresponding to the earlier "Tulip breaking virus lily strain" and "Tulip band breaking virus" isolates. Our newly-determined isolates showed an extremely close relationship to the first of these. The LSV sequence was 8393 nucleotides long and had the typical carlavirus genome organization. The ORF1 protein was most closely related to that of Blueberry scorch virus (57.2% identical amino acids). Sequences of 1796 nt at the 3'-end of three additional LSV isolates from different cultivars were very similar (>98% identical nucleotides) to the corresponding region of the complete sequence. This is the first report of complete sequences for LMoV and LSV.

Identification of Three Potyviruses in Ammi majus in Florida

XII INTERNATIONAL SYMPOSIUM ON VIRUS DISEASES OF ORNAMENTAL PLANTS

Authors: Adkins, S.; Baker, C. A.; Jones, L.; Irey, M. S.; Rosskopf, E. N.

Abstract

Clover yellow vein virus (ClYVV), Bidens mottle virus (BiMoV) and Apium virus Y (ApVY) were identified in Ammi majus in south Florida cut flower production areas in 2005 and/or 2007. To the best of our knowledge, these results are the first report of ClYVV and BiMoV infection of A. majus in North America and the first report of ApVY in North America. Common weeds in Florida are known reservoir hosts for ClYVV including white clover (Trifolium repens) and for BiMoV including Spanish needles (Bidens pilosa) and tropical soda apple (Solanum viarum). Detection of ClYVV and BiMoV in other ornamental crops in Florida suggests that these viruses pose a broader risk to ornamental production.