The Caspase 10 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Caspase 10 protein expression profile in cells. The kit can be used for measuring the relative amounts of Caspase 10 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Caspase 10.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 1 plate 2. 10x TBS: 24 mL (10x), Clear 3. Quenching Buffer: 24 mL (1x), Clear 4. Blocking Buffer: 50 mL (1x), Clear 5. 10x Wash Buffer: 50 mL (10x), Clear 6. 100x Anti-Caspase 10 Antibody (Rabbit Polyclonal): 60 μL (100x), Purple 7. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green 8. HRP-Conjugated Anti-Rabbit IgG Antibody: 6 mL (1x), Glass 9. HRP-Conjugated Anti-Mouse IgG Antibody: 6 mL (1x), Glass 10. Primary Antibody Diluent: 12 mL (1x), Clear 11. Ready-to-Use Substrate: 12 mL (1x), Brown 12. Stop Solution: 12 mL (1x), Clear 13. Crystal Violet Solution: 6 mL (1x), Glass 14. SDS Solution: 24 mL (1x), Clear 15. Adhesive Plate Seals: 4 seals
Storage
4°C/6 Months
Citations
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Tadaki, H; Saitsu, H; et al. Exonic deletion of CASP10 in a patient presenting with systemic juvenile idiopathic arthritis, but not with autoimmune lymphoproliferative syndrome type IIa. INTERNATIONAL JOURNAL OF IMMUNOGENETICS 38:287-293(2011).
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