Cathepsin D ELISA Kit (DEIA-XY100)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
cell culture supernatants, serum, plasma
Species Reactivity
Human
Intended Use
Enzyme-linked immunosorbent assay (ELISA) for the determination of Cathepsin D in cell culture supernatants, serum, or plasma.
Contents of Kit
1. 96-well plate coated with anti-human Cathepsin D antibody: 12 x 8 strips
2. Human Cathepsin D standard: 2 x 10 ng
3. Biotin-labeled detection antibody (100x): 120 μL
4. Streptavidin-HRP (100x):120 μL
5. Standard/Sample diluent:30 mL
6. Detection antibody diluent: 12 mL
7. Streptavidin-HRP diluent: 12 mL
8. Wash buffer (20x): 30 mL
9. TMB substrate solution: 12 mL
10. Stop solution: 12 mL
11. Plate adhesive strips: 3 Strips
12. Technical manual: 1 Manual
Storage
2-8°C
Detection Range
156 pg/mL-10,000 pg/mL
Sensitivity
The minimum detectable dose of this Cathepsin D ELISA typically is less than 10 pg/mL.
Standard Curve

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References


Martinet, W; Schrijvers, DM; et al. Immunohistochemical analysis of macroautophagy Recommendations and limitations. AUTOPHAGY 9:386-402(2013).
Spisak, S; Galamb, O; et al. Downregulated cathepsin D (CTSD) and upregulated E2F2 transcription factor are possible progression markers from early to late stage colorectal cancer determined by antibody and tissue microarrays. GASTROENTEROLOGY 134:A446-A446(2008).

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