CRMP2 (Phospho-Thr509) ELISA Kit

Sample

Cultured cells

Species Reactivity

N/A

Detection Method

iELISA

Intended Use

The CRMP2 (Phospho-Thr509) ELISA Kit is a convenient, lysate- free, high throughput and sensitive assay kit that can monitor CRMP2 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated CRMP2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CRMP2 phosphorylation.

Contents of Kit

1. 96-Well Cell Culture Clear-Bottom Microplate: 2 Plates
2. 10x TBS: 24 ml (10x)
3. Quenching Buffer: 24 ml (1x)
4. Blocking Buffer: 50 ml (1x)
5. 15x Wash Buffer: 50 ml (15x)
6. 100x Anti-CRMP2 Antibody (Phospho-Thr509): 60 μl (100x)
7. 100x Anti-CRMP2 Antibody (Rabbit Polyclonal): 60 μl (100x)
8. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μl (100x)
9. HRP-Conjugated Anti-Rabbit IgG Antibody: 12 ml (1x)
10. HRP-Conjugated Anti-Mouse IgG Antibody: 12 ml (1x)
11. Primary Antibody Diluent: 12 ml (1x)
12. Ready-to-Use Substrate: 12 ml (1x)
13. Stop Solution: 12 ml (1x)
14. Crystal Violet Solution: 12 ml (1x)
15. SDS Solution: 24 ml (1x)
16. Adhesive Plate Sealers: 2 Seals

Storage

Upon receipt, the kit should be stored at 4°C. The un-opened kit will be stable for up to 6 months from the date of shipment if stored at 4°C. Diluted Anti-CRMP2 (Phospho-Thr509) Antibody, Anti-CRMP2 Antibody and diluted Anti-GAPDH Antibody can each be stored at 4°C for up to two weeks. HRP-Conjugated Anti-Rabbit IgG Antibody and HRP-Conjugated Anti-Mouse IgG Antibody will be stable at 4°C for up to six months. The SDS Solution should be stored at room temperature or warmed up to room temperature if stored at 4°C.