Rabbit Anti-CHUK monoclonal antibody, clone TO74-13

NF-kappa B activation in response to UV irradiation of HeLa cells or of primary human skin fibroblasts occurs with two overlapping kinetics but totally different mechanisms. Although both mechanisms involve induced dissociation of NF-kappa B from I kappa B alpha and degradation of I kappa B alpha, targeting for degradation and signaling are different. Early I kappa B alpha degradation at 30 min to similar to 6 h is not initiated by UV-induced DNA damage. It does not require I kappa B kinase (IKK), as shown by introduction of a dominant-negative kinase subunit, and does not depend on the presence of the phosphorylatable substrate, I kappa B alpha, carrying serines at positions 32 and 36. Induced I kappa B alpha degradation requires, however, intact N- (positions 1-36) and C-terminal (positions 277-287) sequences. I kappa B degradation and NF-kappa B activation at late time points, 15-20 h after UV irradiation, is mediated through DNA damage-induced cleavage of IL-1 alpha precursor, release of IL-1 alpha and autocrine/paracrine action of IL-1 alpha. Late-induced I kappa B alpha requires the presence of Ser32 and Ser36. The late mechanism indicates the existence of signal transfer from photoproducts in the nucleus to the cytoplasm. The release of the 'alarmone' IL-1 alpha may account for some of the systemic effects of sunlight exposure.

The purpose of this study is to evaluate the post-discharge prognosis ofpregnant women with vaginal bleeding during the first trimester, admitted to the Emergency Maternity at the Kamenge University Hospital (CHUK), according to patients age, amount of bleeding and ultrasound results, We conducted a retrospective descriptive and analytical study of239 medical records ofpatients hospitalized over a period ofsix years from January 2012 to December 2017, In this study, the prognosis ofpregnant women with first-trimester metrorrhagia hospitalized at the CHUK was bad; the majority of them (65,7%) had miscarriage, Amount of bleeding, maternal age below 20 years, or well above or equal to 35 years, were significant risk factors but much more ultrasound detection of trophoblastic detachment, It would be interesting to perform a prospective study to detect the causes of these metrorrhagias and to determine late pregnancy complications,