Human CETN1 ELISA Matched Antibody Pair

Name: Human CETN1 ELISA Matched Antibody Pair
SKU: ABPR-0202
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Species Reactivity

Human

Intended Use

This antibody pair set comes with matched antibody pair to detect and quantify protein level of human CETN1.

General Description

The protein encoded by this gene plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. This encoded protein is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.

Reconstitution And Storage

Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Isolation and characterization of the Cetn1 gene from tufted deer (Elaphodus cephalophus)

BIOCHEMICAL GENETICS

Authors: Zhang, Wen; Cao, Xiangrong; Shen, Quan; Liu, Wei; Ni, Siyang; Hua, Xiuguo; Zhang, Xiran

Abstract

The tufted deer, a species found only in China, has polymorphic sex chromosomes (2n = 46, 47, 48). Centrins are centrosome components in species from yeast to humans. Here, the Cetn1 gene was isolated from the testis cDNA library of tufted deer, and its expression pattern, number of gene copies, and gene structure were studied. To date, for unknown reasons, satisfactory genomic DNA of the tufted deer has not been obtained. In the present study, we extracted high molecular weight genomic DNA from tissues of the tufted deer. Results showed that genomic DNA was isolated successfully and could be used for Southern blotting of the Cetn1 gene. Centrin 1 transcripts were shown to exist in testis and the retina. It has no intron and only a single copy in the genome. Protein sequence analyses indicate that the C-terminal Ser-170, which was shown to be phosphorylated in human centrin, is not conserved except in sequences from the human, chimpanzee, and tufted deer.

Knockdown of CETN1 inhibits breast cancer cells proliferation

JOURNAL OF BUON

Authors: Xu Shuangta; He Yali; Zheng Zhengrong; Zhao Qingquan; Zeng Xiaoshan; Yao fanghui; Waihung Ng; Liang yinghui; Xu Jianhua

Abstract

Purpose: Breast cancer is a multifactorial disease and identification of the genes and proteins regulating breast carcinogenesis is critical in exploring novel targeted therapies. The aim of this study was to investigate the role CETN1 in breast cancer growth and metastasis. Methods: CETN1 expression levels were measured in breast tissue samples by immunohistochemistry (IHC). We selected high CETN1-expressing cell lines from human breast carcinoma cells with metastatic potential. The proliferation of cells was evaluated by MTS assay. Changes in progression of cell division were assessed by cell cycle analysis. Changes in CETN1 levels in breast tumor cells were analysed by Western blot. Results: Our results demonstrated that overexpression of CETN1 occurred mainly in breast cancer cells in which CETN1 expression was low. Knockdown of CETN1 by lentivirus-shRNA significantly inhibited breast cancer cells' proliferation, growth and metastasis. Conclusion: CETN1 is overexpressed in breast cancer tissue and promotes cells'proliferation, tumor growth and metastasis.