Product Overview
293T cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The CERS6 knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
Alternative Names
CERS6; ceramide synthase 6; CERS5; LASS6; longevity assurance homolog 6; LAG1 homolog, ceramide synthase 6
Application Notes
Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Dilution
Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
Concentration
The protein concentration was determined with BCA assay.
Storage
Store at -20°C. Avoid repeated freeze-thaw cycles.
Citations
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Ebel, P; Dorp, KV; et al. Inactivation of Ceramide Synthase 6 in Mice Results in an Altered Sphingolipid Metabolism and Behavioral Abnormalities. JOURNAL OF BIOLOGICAL CHEMISTRY 288:21433-21447(2013).
Ebel, P; Imgrund, S; et al. Ceramide synthase 4 deficiency in mice causes lipid alterations in sebum and results in alopecia. BIOCHEMICAL JOURNAL 461:147-158(2014).
COA
Certificate of Analysis
