Human CAV1 ELISA Kit

Sample

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Species Reactivity

Human

Detection Method

sELISA

Intended Use

For the quantitative measurement of CAV1 in Human biological samples.
This product is intended for research use only.

Contents of Kit

1. CAV1 Microplate 96 Wells (12 × 8 Well strips), -20°C for 6 months
2. CAV1 Lyophilized Standard 2 vials, -20°C for 6 months
3. 100× Biotinylated CAV1 Detector Antibody 1 × 120 μL, -20°C for 6 months
4. 100× Avidin-HRP Conjugate 1 × 120 μL, -20°C for 6 months
5. Sample Diluent 1 × 20 mL. -20°C for 6 months
6. Detector Antibody Diluent 1 × 12 mL, -20°C for 6 months
7. Conjugate Diluent 1 × 12 mL, -20°C for 6 months
8. 25X Wash Buffer 1 × 30 mL, Store at 4°C for 6 months
9. Stop Solution 1 × 10 mL, Store at 4°C for 6 months
10. TMB Substrate 1 × 10 mL, Store at 4°C for 6 months

Storage

Open kit immediately upon receipt. Store components at -20°C (NOTE: exceptions below) for 6 months or until expiration date. Avoid any freeze/thaw cycles.

General Description

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation. Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae.