Anti-Capn1a polyclonal antibody

There are many potential genes that are known to be related to meat production and that can be used for selection to improve meat quality and production include Calpain (CAPN1), Myostatin (MSTN), Calpastatin (CAST), Osteopontin (SPP1) and Thyroglobulin (TG). This study evaluated Zavot (n=60), Anatolian Black (n=59; AB), South Anatolian Red (n=53; SAR), Turkish Gray (n=60; TG) and East Anatolian Red (n=49; EAR) cattle breeds, with the primary goal of investigating DNA polymorphisms of the CAST, TG, SPP1, MSTN and CAPN1 genes. Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) was employed to genotypes. Restriction enzymes revealed polymorphisms with 2 alleles and three genotypes of each CAST, TG, SPP1 and CAPN1 genes were determined in all breeds, while the MSTN locus was found to be monomorphic. Deviation from Hardy-Weinberg equilibrium (HWE) was not observed in the five cattle breeds on SPP1 locus. Significant deviation was observed from HWE in the TG and AB breeds on TG locus. Except for the TG and SAR breeds, genotype frequencies were not consistent with the HWE on CAPN1 locus. Four breeds were found at Hardy-Weinberg equilibrium except AB breed for CAST locus. Consequently, this study has shown that genetic polymorphisms do not exist in the MSTN gene but do exist in the remaining four genes that were examined in five Turkish native cattle breeds.

Meat quality is an important concern for the poultry industry. Domestic geese products obtained from free-range systems usually have rather tough meat, and it is necessary to select them to improve meat tenderness. The relation of the calpain 1 (CAPN1) gene with the post-mortem tenderness process of meat has been demonstrated in several species. Thus, the objective of the present study was to identify polymorphisms in this gene and to perform an association analysis between these polymorphisms and related economic traits in goose raised in the dehesa ecosystem. For the analysis, 50 geese of 3 different subpopulations (20 Embden Anser anser; 20 Toulouse Anser anser; 10 F1 cross) were studied. The experimental protocols were followed complying with principles of animal welfare. A novel SNP was found in the CAPN1 gene, g.68G -> A. This polymorphism was statistically associated with different carcass and meat quality traits such as thigh muscle width (P = 0.020) and the b* 10-day meat colour parameter (P = 0.024) for the global goose population. The association of this gene with meat tenderness (Warner-Bratzler shear force) was confirmed in the case of female individuals of the Toulouse breed (P = 0.043). The results suggest the possibility of using molecular markers in CAPN1 gene as a potential tool for improving carcass and meat quality traits in goose breeding programmes.