Rabbit Anti-Human CAPN1 (N-terminal) Polyclonal antibody

Name: Rabbit Anti-Human CAPN1 (N-terminal) Polyclonal antibody
SKU: DPABH-22077
Rating: 5 (1 reviews)

Rabbit Anti-Human CAPN1 (N-terminal) Polyclonal antibody for WB, IHC

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COA

SDS

Datasheet

Key features and details

Specificity: CAPN1 Antibody detects endogenous levels of total CAPN1.
Host: Rabbit
Application: WB, IHC
Species Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus

Summary

Specifications

Antibody Isotype

IgG

Species Reactivity

Human; Mouse; Rat

Immunogen

A synthesized peptide derived from human CAPN1, corresponding to a region within N-terminal amino acids.

Conjugate

Unconjugated

Applications

Application Notes

WB 1:500-1:2000
IHC 1:50-1:200

General Notes

This product (Catalog No. DPABH-22077) is a rabbit-derived polyclonal antibody raised against human CAPN1. This antibody exhibits cross-reactivity with CAPN1 from mouse and rat. It has been affinity purified by Protein A. Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
DPABH-22077 is suitable for use in WB 1 (500-1:2000), IHC 1 (50-1:200) applications.
DPABH-22077 is supplied as a liquid formulation in Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Target

Entrez Gene ID

823

UniProt ID

P07384

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Investigation on CAST, CAPN1 and CAPN3 porcine gene polymorphisms and expression in relation to post-mortem calpain activity in muscle and meat quality

MEAT SCIENCE

Authors: Gandolfi, G.; Pomponio, L.; Ertbjerg, P.; Karlsson, A. H.; Costa, L. Nanni; Lametsch, R.; Russo, V.; Davoli, R.

Abstract

This study aimed to detect variability in CAST, CAPN1 and CAPN3 porcine genes and to investigate the effect of CAST and CAPN1 polymorphisms on the activity of native and autolyzed mu-calpain and m-calpain, measured from 1 to 72 h post-mortem in Longissimus dorsi (LD) muscle of 30 pigs. Effects of polymorphisms on meat quality parameter such as pH, color and drip loss were also evaluated. Samples carrying CAST EU137105: g.76,872AA genotype showed higher autolyzed mu-calpain activity 24 and 72 h post-mortem, as well as lower drip loss values. Expression of CAST, CAPN1 and CAPN3 was assessed in LD muscles divergent for shear force. Higher CAST and CAPN3 expression was found in LD with high shear force (P<0.2), confirming a direct role for calpastatin but not for calpain 3 in meat tenderization. In conclusion, CAST gene affected post-mortem activation time of calpain and drip loss. (C) 2011 Elsevier Ltd. All rights reserved.

Association of Calpain 1 (CAPN1) and HRSP12 allelic variants in beef cattle with carcass traits

AFRICAN JOURNAL OF BIOTECHNOLOGY

Authors: Hou, Guanyu; Huang, Meng; Gao, Xue; Li, Junya; Gao, Huijiang; Ren, Hongyan; Xu, Shangzhong

Abstract

Calpain 1 (CAPN1) and its activator HRSP12 are evaluated as candidate gene for quantitative trait loci (QTLs) affecting meat tenderness. In this study, SNPs were detected by sequencing in 323 cattle from 9 breeds. The association results showed that the A3553G and T824C loci individually related with marbling and tenderness, and CAPN1/HRSP12 double homozygote and heterozygote/homozygote pairs (AA/TT, AA/CC, AG/TT and GG/TT) had higher marbling score than the other groups. Our findings suggest that polymorphisms in CAPN1 and HRSP12 might be the important genetic factor influencing meat quality in carcass trait.