WB: A dilution 1:2000 of the antibody was an effective probe for 0.5 μg of C. difficile Toxin A subunit. Direct ELISA: The antibody titer, measured at the midpoint, was 1:23000 using a 2 ug coating of C. difficile Toxin, A subunit. We recommend the following for sandwich ELISA (Capture - Detection): CABT-B1970 - CABT-B1084 For example, a 1 μg coating of CABT-B1970 fllowed by exposure to the antigen and a 1:1000 dilution of the goating antisera gives a midpoint of approximately 40 ng/ml. As low as 1 ng/ml of antigen is readily detected using a standard chromogenic substrate.
Target
Alternative Names
tcdA; toxA; Clostridium difficile Toxin A
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