Magic™ Anti-C. difficile Toxin A polyclonal antibody (CABT-B1970)

Chicken Anti-C. difficile Toxin A polyclonal antibody for WB, ELISA, sELISA Datasheet

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Specifications


Host Species
Chicken
Antibody Isotype
IgY
Species Reactivity
C. difficile
Immunogen
Toxin A from Clostridium difficile
Conjugate
unconjugated

Applications


Application Notes
WB: A dilution 1:2000 of the antibody was an effective probe for 0.5 μg of C. difficile Toxin A subunit.
Direct ELISA: The antibody titer, measured at the midpoint, was 1:23000 using a 2 ug coating of C. difficile Toxin, A subunit.
We recommend the following for sandwich ELISA (Capture - Detection):
CABT-B1970 - CABT-B1084
For example, a 1 μg coating of CABT-B1970 fllowed by exposure to the antigen and a 1:1000 dilution of the goating antisera gives a midpoint of approximately 40 ng/ml. As low as 1 ng/ml of antigen is readily detected using a standard chromogenic substrate.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
tcdA; toxA; Clostridium difficile Toxin A

Citations


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References


Na, X; Kim, H; et al. gp96 is a human colonocyte plasma membrane binding protein for Clostridium difficile toxin A. INFECTION AND IMMUNITY 76:2862-2871(2008).
Modi, N; Gulati, N; et al. Differential Binding and Internalization of Clostridium difficile Toxin A by Human Peripheral Blood Monocytes, Neutrophils and Lymphocytes. SCANDINAVIAN JOURNAL OF IMMUNOLOGY 74:264-271(2011).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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