Magic™ Anti-C. difficile Toxin A polyclonal antibody (CABT-B1970)

Chicken Anti-C. difficile Toxin A polyclonal antibody for WB, ELISA, sELISA

Specifications


Host Species
Chicken
Antibody Isotype
IgY
Species Reactivity
C. difficile
Immunogen
Toxin A from Clostridium difficile
Conjugate
Unconjugated

Applications


Application Notes
WB: A dilution 1:2000 of the antibody was an effective probe for 0.5 μg of C. difficile Toxin A subunit.
Direct ELISA: The antibody titer, measured at the midpoint, was 1:23000 using a 2 ug coating of C. difficile Toxin, A subunit.
We recommend the following for sandwich ELISA (Capture - Detection):
CABT-B1970 - CABT-B1084
For example, a 1 μg coating of CABT-B1970 fllowed by exposure to the antigen and a 1:1000 dilution of the goating antisera gives a midpoint of approximately 40 ng/ml. As low as 1 ng/ml of antigen is readily detected using a standard chromogenic substrate.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
tcdA; toxA; Clostridium difficile Toxin A

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


Demarest, SJ; Salbato, J; et al. Structural characterization of the cell wall binding domains of Clostridium difficile toxins A and B; Evidence that Ca2+ plays a role in toxin A cell surface association. JOURNAL OF MOLECULAR BIOLOGY 346:1197-1206(2005).
Martin, CE; Broecker, F; et al. Immunological Evaluation of a Synthetic Clostridium difficile Oligosaccharide Conjugate Vaccine Candidate and Identification of a Minimal Epitope. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 135:9713-9722(2013).

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