Campylobacter jejuni IgG ELISA Kit

Sample

Serum or plasma (EDTA, citrate, heparin).

Species Reactivity

Human

Detection Method

iELISA

Intended Use

The Campylobacter jejuni IgG tests is qualitative immunoassays for the demonstration of human antibody classes IgG directed against Campylobacter jejuni. The assays are recommended for the sensitive detection of such antibodies in various kinds of samples.

Contents of Kit

1. Break apart microtiter test strips each with eight antigen coated single wells, 12 pieces, (altogether 96):
The coating material is inactivated.
2. Standard serum (ready-to-use), 2 × 2 ml:
Human serum in protein containing phosphate buffer;
negative for anti-HIV Ab, HBs-Ag (Hepatitis B-Virus surface antigen) and anti-HCV Ab;
preservative: < 0.1 % sodium azide;
colouring: Amaranth O.
3. Negative control serum (ready-to-use), 2 ml:
Human serum in protein containing phosphate buffer;
negative for anti-HIV Ab, HBs-Ag (Hepatitis B-Virus surface antigen) and anti-HCV Ab;
preservative: < 0.1 % sodium azide;
colouring: Lissamin Green V.
4. Anti-human IgG conjugate (ready-to-use), 13 ml:
Anti-human IgG polyclonal antibody,
conjugated to alkaline phosphatase, stabilised with protein stabilisation solution;
preservative: 0.01 % methylisothiazolone, 0.01 % bromnitrodioxane.
5. Washing solution concentrate (sufficient for 1000 ml), 33.3ml:
Sodium chloride solution with Tween 20 and 30 mM Tris/HCl, pH 7,4;
preservative: < 0.1 % sodium azide.
6. Dilution buffer (ready to use), 2 × 50 ml:
Protein containing phosphate buffer with Tween 20;
preservative: < 0.1 % sodium azide;
colouring: 0.01 g/l Bromphenol blue.
7. Stopping solution (ready to use), 15 ml:
< 0.1 N sodium hydroxide, 40 mM EDTA
8. Substrate (ready-to-use), 13 ml:
Para-nitrophenylphosphate in solvent free buffer;
preservative: < 0.1 % sodium azide
9. Quality control certificate, 1 page

Storage

Sensitivity

The sensitivity exceeded 99%.

General Description

Worldwide there are more than 20 different Campylobacter species. In the industrialised countries Campylobacter jejuni is the second most common cause of intestinal inflammation. The primary reservoirs for the organisms are wild warm blooded animals and farm animals. Campylobacter infections in humans are primarily a result of food contamination, inadequately heated poultry being the primary source of infection. The incubation time is generally between two and seven days. ELISA test-systems are especially suited for the differential analysis of immunoglobulin classes directed against the virus.