Sample
serum, plasma, and other biological samples
Intended Use
In vitro quantitative determination of B2M concentrations in serum, plasma, cellculture supernatant and other biological samples.
Contents of Kit
The sealed kit can be stored at 2-8°C. The storage condition for opened kit is specified in the table below
1. ELISA Microplate(Dismountable), 8×12. Put the rest strips into a sealed foilbag with the desiccant. Stored for 1month at 2-8°C. Stored for 12 monthat -20°C
2. Lyophilized Standard, 2vial. Put the rest standards into adesiccant bag. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C
3. Biotin-labeledAntibody(Concentrated, 100×), 120μl. 2-8°C (Avoid Direct Light)
4. HRP-StreptavidinConjugate(SABC, 100×), 120μl. 2-8°C (Avoid Direct Light)
5. TMB Substrate, 10ml. 2-8°C (Avoid Direct Light)
6. Sample Dilution Buffer, 20ml. 2-8°C
7. Antibody Dilution Buffer, 10ml. 2-8°C
8. SABC Dilution Buffer, 10ml. 2-8°C
9. Stop Solution, 10ml. 2-8°C
10. Wash Buffer(25×), 30ml. 2-8°C
11. Plate Sealer, 5 pieces
12. Product Description, 1 copy
Note: The liquid reagent bottle contains slightly more reagent than indicated on the label. Please use pipette accurately measure and do proportional dilution
Storage
2-8°C (for sealed box), please do not freeze! See kit label for expiry date
Performance Characteristics
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Detection Range
1.563-100ng/ml
General Description
Beta2-microglobulin is present on the surface of most cells and in the blood. The normal range of beta2-microglobulin in the blood can vary depending on factors such as age, gender, and other factors. Beta2-microglobulin levels can increase in various conditions, including kidney dysfunction, certain types of cancer,and autoimmune disorders, among others. In healthy adults, the normal range of beta2-microglobulin in the blood is between 0.7 - 2.2 mg/L
Standard Curve
This product has been tested by Quality Control Department and meets performance specifications mentioned in the manual. (The humidity in the laboratory is 20%-60%, and the temperature is 18°C - 25°C. TMB was balanced to 37°C before color development, and incubated at 37°C for 15 minutes in the darkafter adding the enzyme label plate holes.)
The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.
Citations
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