Recombinant Profilin-1 produced in E.coli is a non-glycosylated, polypeptide chain having a calculated molecular mass of 15,625 Dalton.
Bet v 2.0101 is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Tested by LAL (Limulus Amoebocyte Lysate) chromogenic endotoxin assay.
Profilin-1; Allergen Bet v II; Pollen allergen Bet v 2; Bet v 2; BETVII; Bet v 2.0101
> 80%, based on SDS PAGE
Batch dependent - please inquire should you have specific requirements.
Neutral to slightly alkaline pH and 20% glycerol as cryoprotective agent.
-70°C or below. Repeated freeze/thaw cycles should be avoided.
Profilin-1 (Bet v 2.0101) binds to actin and affects the structure of the cytoskeleton. At high concentrations, profiling-1 averts the polymerization of actin, whereas it augments it at low concentrations. By binding to PIP2, Profilin-1 inhibits the creation of IP3 and DG.
Profilin-1;Allergen Bet v II;Pollen allergen Bet v 2;Bet v 2;BETVII;Bet v 2.0101