Anti-BCMV polyclonal antibody [AP]

The bean weevil (Acanthoscelides obtectus Say) is an aggressive post-harvest pest of the common bean (Phaseolus vulgaris L.). Bean common mosaic virus (BCMV) and Bean common mosaic necrosis virus (BCMNV) are important seedborne diseases of dry bean in the Americas and Africa. The development of bean lines that combine resistance to BCMV, BCMNV, and bean weevils can help to reduce yield loss during the growing season and avoid seed damage during storage. AO-1012-29-3-3A, (Reg. No. GP-299, PI 675563) is a multiple virus and bean weevil-resistant bean germplasm line adapted to the humid tropics; it was developed and released cooperatively in 2015 by Sokoine University of Agriculture, Oregon State University, the USDA-ARS, and the University of Puerto Rico. AO-1012-29-3-3A has a determinate growth habit and a dark red kidney seed type similar to the cultivar Rojo, which is commercially acceptable in Tanzania. AO-1012-29-3-3A possesses the I and bc-1(2) genes that confer resistance to BCMV and BCMNV. AO-1012-29-3-3A produced a mean seed yield similar to the checks USLK-1 and 'Badillo' in three trials planted in Puerto Rico. Seed of AO-1012-29-3-3A had greater levels of threonine, proline, analine, valine, lysine, methionine, and crude protein compared with the check cultivar Badillo. AO-1012-29-3-3A should serve as a useful source of resistance to the bean weevil, BCMV, and some pathogroups of BCMNV.

Potyvirus isolates from asparagus bean (Vigna sesguipedalis) plants in Zhejiang province, China, caused either rugose and vein banding mosaic symptoms (isolate R) or severe yellowing (isolate Y) in this host, but were otherwise similar in host range. Both isolates were completely sequenced and shown to be isolates of Bean common mosaic virus (BCMV). The complete sequences were 9992 (R) or 10062 (Y) nucleotides long and shared 91.7% identical nucleotides (93.2% identical amino acids) in their genomes and were more distantly related to the BCMV-Peanut stripe virus sequence (PStV). The isolates were much less similar to one another in the 5'-UTR and the N-terminal region of the P 1 protein. In the P1, isolate Y was closer to PStV (76.1% identical amino acids) than to isolate R (64.8%). Phylogenetic analyses of the coat protein region showed that the new isolates grouped with other isolates from Vigna spp., forming the blackeye cowpea mosaic strain subgroup of BCMV with 94-98% nucleotides (96-99% amino acids) identical to one another and about 90% identity to other BCMV isolates. Other significant subgroupings amongst published BCMV isolates were detected.