BCL10 Knockout Cell Lysate (DAG-KO375)

BCL10 Knockout Cell Lysate from HeLa cell line for anti-BCL10 antibody specificity validation. Datasheet

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Product Overview
HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The BCL10 knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
Nature
Cell Lysate
Alternative Names
BCL10; B-cell CLL/lymphoma 10; B-cell lymphoma/leukemia 10; c E10; CARD containing molecule enhancing NF kB; CARD containing apoptotic signaling protein
Application Notes
Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Dilution
Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
Format
Lyophilized
Concentration
The protein concentration was determined with BCA assay.
Size
100 ug
Buffer
RIPA buffer
Storage
Store at -20°C. Avoid repeated freeze-thaw cycles.

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