Tag/Conjugate
Unconjugated
Molecular Weight
The molecular weight determined by electrospray ionization mass spectrometry is 17.16 kDa.
Alternative Names
Anthrax Protective Antigen;PagA
Format
Lyophilized power, sealed under vacuum
Storage
Store at 2-8°C prior to reconstitution.
Reconstitution
Anthrax toxin proteins may be reconstituted in sterile purified water, stored at 2-8°C and used successfully within a few hours. If it is necessary to store this material, reconstitute it at a concentration of 1 mg/ml. Reconstitution with 1 mg/ml BSA may enhance stability and recovery. It is further recommended that the solution is aliquoted and frozen at either -20°C or -70°C. After the protein has been reconstituted as described above, glycerol may be added to 50% if a liquid is desired at freezer temperatures. Storage of material reconstituted in BSA at 2-8°C for a period of two weeks may be acceptable for some applications.
Citations
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Shcherbinin, DN; Esmagambetov, IB; et al. Protective Immune Response against Bacillus anthracis Induced by Intranasal Introduction of a Recombinant Adenovirus Expressing the Protective Antigen Fused to the Fc-fragment of lgG2a. ACTA NATURAE 6:76-84(2014).
Albrecht, MT; Livingston, BD; et al. Electroporation of a multivalent DNA vaccine cocktail elicits a protective immune response against anthrax and plague. VACCINE 30:4872-4883(2012).
COA
Certificate of Analysis
