Alternative Names
Anthrax Protective Antigen;PagA
Format
Lyophilized power, sealed under vacuum
Storage
Store at 4°C prior to and following reconstitution. This product is light sensitive.
Reconstitution
Anthrax toxin proteins may be reconstituted in sterile distilled water, stored at 4°C and used successfully within a few hours. However, over longer periods of time, there may be a decline in the PA binding. If it is necessary to store this material, reconstitute it at a concentration of 1 mg/ml. Reconstitution with 1 mg/ml BSA may enhance stability and recovery. It is further recommended that the solution is aliquoted and frozen at either -20°C or -70°C. After the protein has been reconstituted as described above, glycerol may be added to 50% if a liquid is desired at freezer temperatures. Storage of material reconstituted in BSA at 4°C for a period of two weeks may be acceptable for some applications.
Citations
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Cohen, S; Mendelson, I; et al. Attenuated nontoxinogenic and nonencapsulated recombinant Bacillus anthracis spore vaccines protect against anthrax. INFECTION AND IMMUNITY 68:4549-4558(2000).
Reed, CD; Rast, H; et al. Expression of furin-linked Fab fragments against anthrax toxin in a single mammalian expression vector. PROTEIN EXPRESSION AND PURIFICATION 54:261-266(2007).
COA
Certificate of Analysis
