Anti-CGB monoclonal antibody, clone P1G7F12 (BIO-BCG-005)

Cemented gangue backfill (CGB, a mixture of coal gangue, cement and water) is widely used in China for backfilling in coal mines. The addition of fly ash to CGB mixtures can help them to have favourable environmental and economic performance. In addition, mechanical stability and durability are also important performance criteria for CGB, and these two criteria are closely related to the suction of CGB. The evolution of suction in CGB is influenced by binder hydration, which is an exothermic process affecting the variation in temperature of CGB. To examine the coupled effects of binder hydration and temperature (including the temperatures of a CGB mixture and its surroundings), in this study a numerical model was developed to predict the development of suction in a CGB mixture containing fly ash. Data from a laboratory study were used to validate the developed model, and it was found that the results of the model simulation and the experimental study were in good agreement. Therefore, the proposed model was applied to simulate and determine the development of suction within the CGB mixture over curing time. The results obtained can contribute to better design and preparation of CGB mixtures, as well as better prediction of the evolution of suction within CGB structures.

Consistent with its role as a nitric oxide (NO)-detoxifying globin in Campylobacter jejuni, Cgb (Campylobacter globin) expression is strongly and specifically induced following exposure to nitrosative stress, suggesting a previously unrecognized capacity for NO-related stress sensing in this food-borne pathogen. In this study, Fur and PerR have been eliminated as major regulators of cgb, and NssR (Cj0466), a member of the Crp-Fnr superfamily, has been identified as the major positive regulatory factor that controls nitrosative stress-responsive expression of this gene. Accordingly, disruption of nssR resulted in the abolition of inducible cgb expression, which was restored by a complementing chromosomal insertion of the wild-type gene with its indigenous promoter at a second location. The NssR-deficient mutant was more sensitive to NO-related stress than a cgb mutant and this phenotype most likely arises from the failure of these cells to induce other NO-responsive components in addition to Cgb. Indeed, analysis of global gene expression, by microarray and confirmatory real-time polymerase chain reaction (PCR) in the wild type and nssR mutant, not only confirmed the dependence of inducible cgb expression on NssR, but also revealed for the first time a novel NssR-dependent nitrosative stress-responsive regulon. This regulon of at least four genes includes Cj0465c, a truncated globin. Consistent with NssR being a Crp-Fnr superfamily member, an Fnr-like binding sequence (TTAAC-N-4-GTTAA) was found upstream of each gene at locations -40.5 to -42.5 relative to the centre of the binding sites and the transcription start point. Site-directed mutagenesis confirmed that this cis-acting motif mediates the nitrosative stress-inducible expression of cgb.