Rabbit Anti-Human YOD1 (a.a. 63-348) Polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Human

Immunogen

Recombinant human YOD1 protein; Source: E. coli derived, PET28a; Tag: 6*His; Domain: 63-348 aa of BC137167

Conjugate

Application Notes

WB: 1:500-1:2000
IP: 0.5-4.0 μg for 1.0-3.0 mg of total protein lysate
IHC: 1:100-1:400

General Notes

This product (Catalog No. DPABH-20302) is a rabbit-derived polyclonal antibody raised against human YOD1, targeting the amino acid region 63–348 of the protein. It has been affinity purified by Protein A. Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCPs axial channel. Mediates deubiquitination of both Lys-48- and Lys-63-linked polyubiquitin chains. Able to cleave both polyubiquitin and di-ubiquitin.
DPABH-20302 is suitable for use in Western blot (WB) (1:500–1:2,000), IP (0.5-4.0 μg for 1.0-3.0 mg of total protein lysate), IHC (1:100–1:400) applications.
DPABH-20302 is supplied as a liquid formulation in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.

Alternative Names

DUBA 8; DUBA8; HIN 7; HIN7; HIV 1 induced protease 7; HsHIN7; OTUD2; PRO0907; Ubiquitin thioesterase OTU1; YOD1

Entrez Gene ID

UniProt ID