Anti-XPO5 monoclonal antibody, clone FQS9563

Antibody Isotype

IgG

Clone

FQS9563

Species Reactivity

Mouse; Rat; Human

Immunogen

A synthetic peptide corresponding to residues in Human Exportin-5.

Conjugate

Application Notes

WB: 1/1000 - 1/10000; IHC-P: 1/250 - 1/500; Flow Cyt: 1/10 - 1/100; ICC/IF: 1/50 - 1/100.

Alternative Names

XPO5; exportin 5; exportin-5; KIAA1291; ran-binding protein 21; exp5; FLJ14239; FLJ32057; FLJ45606;

Entrez Gene ID

UniProt ID

Antigen Description

Mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP-and GDP-bound forms of Ran between the cytoplasm and nucleus. Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi).Mediates the nuclear export of micro-RNA precursors, which form short hairpins. Also mediates the nuclear export of synthetic short hairpin RNAs used for RNA interference, and adenovirus VA1 dsRNA. In some circumstances can also mediate the nuclear export of deacylated and aminoacylated tRNAs. Specifically recognizes dsRNAs that lack a 5-overhang in a sequence-independent manner, have only a short 3-overhang, and that have a double-stranded length of at least 15 base-pairs. Binding is dependent on Ran-GTP.

Pathway

Gene Expression, organism-specific biosystem; MicroRNA (miRNA) Biogenesis, organism-specific biosystem; RNA transport, organism-specific biosystem; RNA transport, conserved biosystem; Regulatory RNA pathways, organism-specific biosystem;