Anti-VCA EBV IgA ELISA Kit

Sample

Serum

Species Reactivity

Human

Detection Method

iELISA

Intended Use

Anti-VCA EBV IgA ELISA Kit for the detection of IgA antibodies to Epstein Barr virus (EBV) capsid antigen (VCA) in human serum.

Contents of Kit

ELISA break-away strips coated with specific antigen STRIPSAg 1 microplate
1. 1.3 mL Calibrator r.t.u.¹⁾ CAL 1 vial
2. 1.3 mL Positive control serum r.t.u. PC 1 vial
3. 1.3 mL Negative control serum r.t.u. NC 1 vial
4. 13 mL Anti-Human IgA antibodies labelled with horseradish peroxidase r.t.u (Px-conjugate) CONJ 1 vial
5. 55 mL Wash buffer 10x conc. WASH 10x 1 vial
6. 60 mL Dilution buffer r.t.u. DIL 1 vial
7. 13 mL TMB substrate r.t.u. TMB 1 vial
8. 13 mL Stop solution r.t.u. STOP 1 vial
9.Instruction manual
10.Certificate of quality
¹⁾ ready to use
Notice: Control sera may be colorless to yellowish or blue due to the use of different diluents.
Chromogenic substrate TMB is compatible and interchangeable between ELISA kits which contain TMB and not with other Chromogenic substrates TMB-O, TMB-BF.

Storage

The ELISA kit should be used within three months after opening.
Store the kit and the kit reagents at +2 to +10°C, in a dry place and protected from the light. Store unused strips in the sealable pouch and keep the desiccant inside.
Store serum samples at +2 to +10°C up to one week. For longer period make aliquots and keep them at -18°C to -28°C. Avoid repeated thawing and freezing.
Do not store diluted serum samples. Always prepare fresh.
Kits are shipped in cooling bags, the transport time of 72 hours have no influence on expiration. If you find damage at any part of the kit, please inform the manufacturer immediately.
Expiration date is indicated at the ELISA kit label and at all reagent labels.

Precision

The intraassay variability (within the test) and the interassay variability (between tests) were performed with samples of variable absorbance values.
Intraassay variability
The coefficient of intraassay variability is max. 8%. It is measured for each particular Lot at least on 12 parallels of the same microtitration plate.
Example: (N = number of parallels of the same microtitration plate, ±σ = standard deviation)

Interassay variability
The coefficient of interassay variability is max. 15%. It is measured for each particular Lot as comparison of the OD values of the same serum sample in several consecutive tests.
Example: (N = number of an independent examination of the same serum sample, ±σ= standard deviation):